Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation

Emily Baechler Gillespie, F. M. Batliwalla, George Karypis, P. M. Gaffney, K. Moser, W. A. Ortmann, K. J. Espe, S. Balasubramanian, K. M. Hughes, J. P. Chan, A. Begovich, S. Y.P. Chang, P. K. Gregersen, T. W. Behrens

Research output: Contribution to journalArticle

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Abstract

Monitoring of gene and protein expression in peripheral blood cells has significant potential for improving the diagnosis and therapy of many human diseases. As genomic-scale microarray and proteomic technologies are applied to peripheral blood, it is important to consider the variables that may affect interpretation of data. Here we report experiments performed to identify genes that are particularly sensitive to ex vivo handling prior to RNA extraction for gene expression microarrays or quantitative real-time RT-PCR assays. We examined Affymetrix gene expression in samples from eight normal individuals where blood was processed for RNA either immediately after blood draw or the next day following overnight incubation. These studies identified hundreds of genes that are sensitive to ex vivo handling of blood, and suggest that this is an important variable to consider when designing and interpreting human PBMC experiments.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalGenes and Immunity
Volume5
Issue number5
DOIs
StatePublished - Aug 1 2004

Keywords

  • Gene expression
  • Microarray
  • Peripheral blood mononuclear cells
  • RNA
  • Stress response

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    Baechler Gillespie, E., Batliwalla, F. M., Karypis, G., Gaffney, P. M., Moser, K., Ortmann, W. A., Espe, K. J., Balasubramanian, S., Hughes, K. M., Chan, J. P., Begovich, A., Chang, S. Y. P., Gregersen, P. K., & Behrens, T. W. (2004). Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation. Genes and Immunity, 5(5), 347-353. https://doi.org/10.1038/sj.gene.6364098