Expression and x-ray structural determination of the nucleoprotein of lassa fever virus

Xiaoxuan Qi; Wenjian Wang; Haohao Dong; Yuying Liang; Changjiang Dong; Hinh Ly

doi:10.1007/978-1-4939-6981-4_12

Expression and x-ray structural determination of the nucleoprotein of lassa fever virus

Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Dong, Hinh Ly

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	179-188
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-6981-4_12
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1604
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media LLC 2018.

Keywords

Arenavirus
Large-scale expression
Lassa virus
Nucleoprotein
X-ray structure

Publisher link

10.1007/978-1-4939-6981-4_12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351422

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abstract = "We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 {\AA}. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 {\AA}, c = 56.40 {\AA}, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.",

keywords = "Arenavirus, Large-scale expression, Lassa virus, Nucleoprotein, X-ray structure",

author = "Xiaoxuan Qi and Wenjian Wang and Haohao Dong and Yuying Liang and Changjiang Dong and Hinh Ly",

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T1 - Expression and x-ray structural determination of the nucleoprotein of lassa fever virus

AU - Qi, Xiaoxuan

AU - Wang, Wenjian

AU - Dong, Haohao

AU - Liang, Yuying

AU - Dong, Changjiang

AU - Ly, Hinh

N1 - Publisher Copyright: © Springer Science+Business Media LLC 2018.

PY - 2018

Y1 - 2018

N2 - We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

AB - We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

KW - Arenavirus

KW - Large-scale expression

KW - Lassa virus

KW - Nucleoprotein

KW - X-ray structure

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Expression and x-ray structural determination of the nucleoprotein of lassa fever virus

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