Abstract
We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.
Original language | English (US) |
---|---|
Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 179-188 |
Number of pages | 10 |
DOIs | |
State | Published - 2018 |
Publication series
Name | Methods in Molecular Biology |
---|---|
Volume | 1604 |
ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media LLC 2018.
Keywords
- Arenavirus
- Large-scale expression
- Lassa virus
- Nucleoprotein
- X-ray structure