Abstract
Beta-catenin is not only an adhering junction protein, but also the central player of the canonical Wnt signalling pathway. In order to investigate the roles of β-catenin in the mechanism of myocardial hypertrophy, we determined the expression and distribution of β-catenin in the cardiomyocytes of spontaneously hypertensive heart failure (SHHF) rats and age-matched Wistar-Kyoto (WKY) rats. We identified the reducing of β-catenin expression in the membrane protein fraction but increasing in the nuclear protein in the 6 and 12 month-old SHHF rats as compared with the age-matched WKY rats by Western blotting. Immunolabeling of β-catenin colocalized with cadherin at the intercalated disc sites and showed nuclear accumulation in myocytes of SHHF rats. We also revealed that the association between glycogen synthase kinase-3β and β-catenin had weakened in the 6 month-old SHHF rats as compared with the age-matched WKY rats by immunoprecipitation. These findings suggested that nuclear translocation of β-catenin might play important roles in regulating signal transduction in the decompensated hypertrophy stage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 565-573 |
| Number of pages | 9 |
| Journal | Journal of molecular histology |
| Volume | 44 |
| Issue number | 5 |
| DOIs | |
| State | Published - Oct 2013 |
Bibliographical note
Funding Information:This work was supported by the National Natural Science Foundation of China (30871046) and the Guangdong Natural Science Foundation (8151008901000162).
