Expression and function of TLR2, TLR4, and Nod2 in primary canine colonic epithelial cells

Mathew P. Swerdlow, Douglas R. Kennedy, Jeffrey S. Kennedy, Robert J. Washabau, Paula S. Henthorn, Peter F. Moore, Simon R. Carding, Peter J. Felsburg

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The gut maintains a delicate balance between the downregulation of inflammatory reactions to commensal bacteria and the capacity to respond to pathogens with vigorous cellular and humoral immune responses. Intestinal epithelial cells, including colonic epithelial cells (CECs) possess many properties of cells of the innate immune system, in particular the ability to recognize and respond to microbial antigens. Recognition of microorganisms by CECs is based upon their recognition of signature molecules, called microbe-associated molecular patterns (MAMP), by pattern recognition receptors (PRR) including membrane toll-like receptors (TLR) and cytosolic Nod2, an intracellular counterpart of TLRs. The purpose of this study was to determine whether primary CECs from normal dogs express a functional TLR2, TLR4, and Nod2 and whether they are regulated by inflammatory mediators. We show that canine primary CECs express TLR2, TLR4, and Nod2 that can be modulated in response to their respective MAMPs, lipopolysaccharides (LPS) or peptidoglycans (PGN). Furthermore, we demonstrate that these receptors are functional as evidenced by the induction of cytokine gene expression in response to LPS or PGN.

Original languageEnglish (US)
Pages (from-to)313-319
Number of pages7
JournalVeterinary immunology and immunopathology
Volume114
Issue number3-4
DOIs
StatePublished - Dec 15 2006

Fingerprint

Canidae
epithelial cells
Epithelial Cells
dogs
Peptidoglycan
peptidoglycans
lipopolysaccharides
Lipopolysaccharides
microorganisms
Pattern Recognition Receptors
receptors
Toll-Like Receptors
Humoral Immunity
humoral immunity
Cellular Immunity
cell-mediated immunity
Immune System
cytokines
Down-Regulation
digestive system

Keywords

  • Dog
  • Epithelial cells
  • Innate immunity
  • Intestine
  • Toll-like receptor

Cite this

Swerdlow, M. P., Kennedy, D. R., Kennedy, J. S., Washabau, R. J., Henthorn, P. S., Moore, P. F., ... Felsburg, P. J. (2006). Expression and function of TLR2, TLR4, and Nod2 in primary canine colonic epithelial cells. Veterinary immunology and immunopathology, 114(3-4), 313-319. https://doi.org/10.1016/j.vetimm.2006.09.003

Expression and function of TLR2, TLR4, and Nod2 in primary canine colonic epithelial cells. / Swerdlow, Mathew P.; Kennedy, Douglas R.; Kennedy, Jeffrey S.; Washabau, Robert J.; Henthorn, Paula S.; Moore, Peter F.; Carding, Simon R.; Felsburg, Peter J.

In: Veterinary immunology and immunopathology, Vol. 114, No. 3-4, 15.12.2006, p. 313-319.

Research output: Contribution to journalArticle

Swerdlow, MP, Kennedy, DR, Kennedy, JS, Washabau, RJ, Henthorn, PS, Moore, PF, Carding, SR & Felsburg, PJ 2006, 'Expression and function of TLR2, TLR4, and Nod2 in primary canine colonic epithelial cells', Veterinary immunology and immunopathology, vol. 114, no. 3-4, pp. 313-319. https://doi.org/10.1016/j.vetimm.2006.09.003
Swerdlow, Mathew P. ; Kennedy, Douglas R. ; Kennedy, Jeffrey S. ; Washabau, Robert J. ; Henthorn, Paula S. ; Moore, Peter F. ; Carding, Simon R. ; Felsburg, Peter J. / Expression and function of TLR2, TLR4, and Nod2 in primary canine colonic epithelial cells. In: Veterinary immunology and immunopathology. 2006 ; Vol. 114, No. 3-4. pp. 313-319.
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