Exposure to Dimethyl Selenie (DMSe)-Derive Seconary Organic Aerosol Alters Transcriptomic Profiles in Human Airway Epithelial Cells

C. M.Sabbir Ahme, Yumeng Cui, Alexaner L. Frie, Abigail Burr, Rohan Kamath, Jin Y. Chen, Arafat Rahman, Tara M. Norgren, Ying Hsuan Lin, Roya Bahreini

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Dimethyl selenie (DMSe) is one of the major volatile organoselenium compouns release from aquatic an terrestrial environments through microbial transformation an plant metabolism. The etaile processes of DMSe leaing to seconary organic aerosol (SOA) formation an the pulmonary health effects inuce by inhalation of DMSe-erive SOA remain largely unknown. In this stuy, we characterize the chemical composition an formation yiels of SOA prouce from the oxiation of DMSe with OH raicals an O3 in controlle chamber experiments. Further, we profile the transcriptome-wie gene expression changes in human airway epithelial cells (BEAS-2B) after exposure to DMSe-erive SOA. Our analyses inicate a significantly higher SOA yiel resulting from the OH-initiate oxiation of DMSe. The oxiative potential of DMSe-erive SOA, as measure by the ithiothreitol (DTT) assay, suggeste the presence of oxiizing moieties in DMSe-erive SOA at levels higher than typical ambient aerosols. Utilizing RNA sequencing (RNA-Seq) techniques, gene expression profiling followe by pathway enrichment analysis reveale several major biological pathways perturbe by DMSe-erive SOA, incluing elevate genotoxicity, DNA amage, an p53-meiate stress responses, as well as ownregulate cholesterol biosynthesis, glycolysis, an interleukin IL-4/IL-13 signaling. This stuy highlights the significance of DMSe-erive SOA as a stressor in human airway epithelial cells.

Original languageEnglish (US)
Pages (from-to)14660-14669
Number of pages10
JournalEnvironmental Science and Technology
Volume53
Issue number24
DOIs
StatePublished - Dec 17 2019
Externally publishedYes

Bibliographical note

Funding Information:
The authors gratefully acknowledge support from USDA-NIFA Hatch (accession no. 1015963, project no. CA-R-ENS-5072-H) and assistance from Donna Sueper (Aerodyne Research Inc.) for the high-resolution selenium analysis of the mAMS spectra. The authors also thank Alexa Canchola for her experimental assistance.

Publisher Copyright:
Copyright © 2019 American Chemical Society.

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