Exposure to antimicrobials through the milk diet or systemic therapy is associated with a transient increase in antimicrobial resistance in fecal Escherichia coli of dairy calves

C. A. Foutz, S. M. Godden, J. B. Bender, F. Diez-Gonzalez, M. Akhtar, A. Vatulin

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3 Scopus citations

Abstract

The objective of this prospective cohort study was to describe the relationship between exposure to antimicrobials, through both the milk diet and systemic therapy, and to describe antimicrobial resistance of fecal Escherichia coli in dairy calves pre- and postweaning. A convenience sample of 15 Minnesota dairy farms was chosen, representing 3 equal cohorts of milk diet fed to preweaned calves: medicated milk replacer (MMR), nonmedicated milk replacer (NMR), or pasteurized nonsaleable milk (PNM). Five newborn calves were enrolled on each farm, with fecal samples collected from each calf at 1, 3, 5, and 16 wk of age. After isolation, 3 colonies of E. coli were randomly selected from each sample to determine antimicrobial susceptibility by minimum inhibitory concentration (Sensititer, Thermo Scientific, Waltham, MA) to 8 antimicrobials in 8 classes. The isolate was given an antimicrobial resistance score (ARS) according to the number of antimicrobial classes to which it was resistant. Any isolate resistant to 3 or more antimicrobials was defined as being multidrug resistant (MDR). Relationships between ARS and MDR (dependent variables) and possible explanatory variables were analyzed using mixed multivariable linear and logistic regression models, respectively, with critical P-values adjusted for multiple contrasts. Seventy percent of isolates were resistant to sulfadimethoxine. For wk 1 and 3, the mean ARS values were greatest for fecal E. coli from calves fed MMR or PNM compared with NMR, with no difference in ARS values between the MMR and PNM groups at either time point. At wk 5, the mean ARS value was greatest for fecal E. coli from calves fed MMR (3.56 ± 0.45; mean ± SE), intermediate for calves fed PNM (2.64 ± 0.45), and lowest for calves fed NMR (1.54 ± 0.45). However, by wk 16, the mean ARS values were ≤1.0 and did not differ among milk diets. Evaluation of the proportion of isolates with MDR mirrored the results of the ARS analysis (MDR more prevalent in MMR and PNM groups preweaning; no difference among milk diets at 16 wk). There was a tendency for an increase in ARS at wk 5 (1.28 ± 0.70), and the odds for MDR in fecal E. coli were estimated to be 5.2 (95% confidence interval = 0.67, 35.7) and 101.1 (95% confidence interval = 1.15, >999.9) higher at wk 3 and 5 if the calf was treated with a systemic antimicrobial within the 14-d period before sampling. These findings suggest that exposure to antimicrobials through the milk diet or systemic therapy may result in a transient increase in resistance in fecal E. coli, but once the antimicrobial pressure is removed, susceptible E. coli are able to flourish again, resulting in an overall decrease in resistance.

Original languageEnglish (US)
Pages (from-to)10126-10141
Number of pages16
JournalJournal of Dairy Science
Volume101
Issue number11
DOIs
StatePublished - Nov 2018

Bibliographical note

Funding Information:
This work was supported by a grant from the Minnesota Agricultural Experiment Station. We thank the participating dairy producers for allowing us to collect samples from their farms. We also thank various people at the University of Minnesota who contributed in a variety of ways, including Randall Singer for his consulting expertise, Aaron Rendahl for his statistical consulting expertise, Habner Bastos and Cristiano Gontijo for their technical work, Karen Olsen for her expertise and consulting on the MIC testing, and Jenny Timmerman along with the Laboratory for Udder Health staff for their technical support on MIC testing. Finally, we thank Mary Bulthaus from DQCI Laboratory (Mounds View, MN) for her consulting expertise and technical work with analysis of the pasteurized nonsaleable milk samples.

Keywords

  • antimicrobial resistance
  • calf
  • milk
  • therapy

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