Exploring the evolution of multicellularity in Saccharomyces cerevisiae under bacteria environment: An experimental phylogenetics approach

Julian F. Quintero-Galvis, Rocío Paleo-López, Jaiber J. Solano-Iguaran, María Josefina Poupin, Thomas Ledger, Juan Diego Gaitan-Espitia, Andrzej Antoł, Michael Travisano, Roberto F. Nespolo

Research output: Contribution to journalArticlepeer-review

Abstract

There have been over 25 independent unicellular to multicellular evolutionary transitions, which have been transformational in the complexity of life. All of these transitions likely occurred in communities numerically dominated by unicellular organisms, mostly bacteria. Hence, it is reasonable to expect that bacteria were involved in generating the ecological conditions that promoted the stability and proliferation of the first multicellular forms as protective units. In this study, we addressed this problem by analyzing the occurrence of multicellularity in an experimental phylogeny of yeasts (Sacharomyces cerevisiae) a model organism that is unicellular but can generate multicellular clusters under some conditions. We exposed a single ancestral population to periodic divergences, coevolving with a cocktail of environmental bacteria that were inoculated to the environment of the ancestor, and compared to a control (no bacteria). We quantified culturable microorganisms to the level of genera, finding up to 20 taxa (all bacteria) that competed with the yeasts during diversification. After 600 generations of coevolution, the yeasts produced two types of multicellular clusters: clonal and aggregative. Whereas clonal clusters were present in both treatments, aggregative clusters were only present under the bacteria treatment and showed significant phylogenetic signal. However, clonal clusters showed different properties if bacteria were present as follows: They were more abundant and significantly smaller than in the control. These results indicate that bacteria are important modulators of the occurrence of multicellularity, providing support to the idea that they generated the ecological conditions-promoting multicellularity.

Original languageEnglish (US)
Pages (from-to)4619-4630
Number of pages12
JournalEcology and Evolution
Volume8
Issue number9
DOIs
StatePublished - May 2018

Bibliographical note

Funding Information:
This work was funded by FONDECYT grant No. 1130750 and MIISSB Iniciativa Cient?fica Milenio-MINECON. R. Nespolo and MJ. Poupin also thank a CAPES grant FB-0002-2014. A. Anto? thanks POKL.04.01.01-00-053/09 European Social Fund Grant and Institute of Environmental Sciences Jagiellonian University funds DS/WBINOZ/INO?/757/016. J. Quintero-Galvis and J. Solano-Iguaran thank a Conicyt fellowship Doctorado Nacional Chile/2016. Rocio Paleo thanks a Conicyt-fellowship Maestr?a Nacional Chile/2016.

Keywords

  • clonal multicellularity
  • experimental evolution
  • multicellularity
  • social multicellularity
  • yeasts

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