Exploring the Conformation-Regulated function of Titin Kinase by mechanical pump and probe experiments with single molecules

Elias M. Puchner, Hermann E. Gaub

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Figure Presented Feel the force: A new AFM-based singlemolecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins (see picture). It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.

Original languageEnglish (US)
Pages (from-to)1147-1150
Number of pages4
JournalAngewandte Chemie - International Edition
Volume49
Issue number6
DOIs
StatePublished - Feb 1 2010

Keywords

  • Enzymes
  • Force spectroscopy
  • Scanning probe microscopy
  • Sensors
  • Single-molecule studies

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