Exploration of genetic diversity among medicinally important genus Epimedium species based on genomic and EST-SSR marker

Zubaida Yousaf, Weiming Hu, Yanjun Zhang, Shaohua Zeng, Ying Wang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Epimedium species has gained prime importance due to their medicinal and economic values. Therefore, in this study, 26 genomic SSR and 10 EST-SSR markers were developed for 13 medicinal species of the Epimedium genus and one out-group species Vancouveria hexandra W. J. Hooker to explore the existing genetic diversity. A total of 100 alleles by genomic SSR and 65 by EST-SSR were detected. The genomic SSR markers were presented between 2-7 alleles per locus. The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.00 to 4.5 and 0.0254 to 2.8108, respectively. Similarly, for EST-SSR, these values were ranged from 3.00 to 4.00 and 1.9650 to 2.7142. The number of alleles for EST-SSR markers ranged from 3 to 10 with an average of 3.51 per loci. It has been concluded that medicinally important species of the genus Epimedium possesses lower intraspecific genetic variation.

Original languageEnglish (US)
Pages (from-to)1020-1025
Number of pages6
JournalNatural Product Research
Volume29
Issue number11
DOIs
StatePublished - Jun 3 2015

Bibliographical note

Funding Information:
Authors are thankful for the financial support from the Knowledge Innovation Project of the Chinese Academy of Sciences [KSCX2-EW-J-20], the TWAS-CAS Post doc fellowship 2010, the National Natural Science Foundation of China [30900076] and the CAS/SAFEA International Partnership Program for Creative Research Teams Project.

Publisher Copyright:
© 2014 Taylor & Francis.

Keywords

  • Epimedium
  • Systematics
  • genetic diversity
  • genomic SSR/EST SSR markers

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