Experimental study of diffusion-based extraction from a cell suspension

Clara Mata; Ellen K. Longmire; David H. McKenna; Katie K. Glass; Allison Hubel

doi:10.1007/s10404-008-0265-9

Experimental study of diffusion-based extraction from a cell suspension

Clara Mata, Ellen K. Longmire, David H. McKenna, Katie K. Glass, Allison Hubel

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A recently proposed application of microfluidics is the post-thaw processing of biological cells. Numerical simulations suggest that diffusion-based extraction of the cryoprotective agent dimethyl sulfoxide (DMSO) from blood cells is viable and more efficient than centrifugation, the conventional method of DMSO removal. In order to validate the theoretical model used in these simulations, a prototype was built and the flow of two parallel streams, a suspension of Jurkat cells containing DMSO and a wash stream that contained neither cells nor DMSO, was characterized experimentally. DMSO transport in a rectangular channel (depth 500 μm, width 25 mm and overall length 125 mm) was studied as a function of three dimensionless parameters: depth ratio of the streams, cell volume fraction in the cell solution, and the Peclet number (Pe ) based on channel depth, average flow rate and the diffusion coefficient for DMSO in water. In our studies, values of Pe ranged from O(10³) to O(10⁴). Laminar flow was ensured by keeping the Reynolds number between O(1) and O(10). Experimental results based on visual and quantitative data demonstrate conclusively that a microfluidic device can effectively remove DMSO from liquid and cell laden streams without compromising cell recovery. Also, flow conditions in the microfluidic device appear to have no adverse effect on cell viability at the outlet. Further, the results demonstrate that we can predict the amount of DMSO removed from a given device with the theoretical model mentioned previously.

Original language	English (US)
Pages (from-to)	529-540
Number of pages	12
Journal	Microfluidics and Nanofluidics
Volume	5
Issue number	4
DOIs	https://doi.org/10.1007/s10404-008-0265-9
State	Published - 2008

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the University of Minnesota Grant-in-aid Program, the National Blood Foundation and the National Institutes of Health (Grant No. R21EB004857). We would like to thank Brian Darr for his invaluable help and Dave Hultman, from the Research Shop at the Department of Electrical and Computer Engineering for helping with the design and fabrication of the microfluidic device. Anthony Cacace is acknowledged for helping with the exploded view of the channel.

Keywords

Cell suspension
Channel flow
DMSO-extraction
Diffusion
Microfluidics

Publisher link

10.1007/s10404-008-0265-9

Find It

Find It at U of M Libraries

Cite this

@article{911a8c2979bd4c69842c6e04ae8c9790,

title = "Experimental study of diffusion-based extraction from a cell suspension",

abstract = "A recently proposed application of microfluidics is the post-thaw processing of biological cells. Numerical simulations suggest that diffusion-based extraction of the cryoprotective agent dimethyl sulfoxide (DMSO) from blood cells is viable and more efficient than centrifugation, the conventional method of DMSO removal. In order to validate the theoretical model used in these simulations, a prototype was built and the flow of two parallel streams, a suspension of Jurkat cells containing DMSO and a wash stream that contained neither cells nor DMSO, was characterized experimentally. DMSO transport in a rectangular channel (depth 500 μm, width 25 mm and overall length 125 mm) was studied as a function of three dimensionless parameters: depth ratio of the streams, cell volume fraction in the cell solution, and the Peclet number (Pe ) based on channel depth, average flow rate and the diffusion coefficient for DMSO in water. In our studies, values of Pe ranged from O(103) to O(104). Laminar flow was ensured by keeping the Reynolds number between O(1) and O(10). Experimental results based on visual and quantitative data demonstrate conclusively that a microfluidic device can effectively remove DMSO from liquid and cell laden streams without compromising cell recovery. Also, flow conditions in the microfluidic device appear to have no adverse effect on cell viability at the outlet. Further, the results demonstrate that we can predict the amount of DMSO removed from a given device with the theoretical model mentioned previously.",

keywords = "Cell suspension, Channel flow, DMSO-extraction, Diffusion, Microfluidics",

author = "Clara Mata and Longmire, {Ellen K.} and McKenna, {David H.} and Glass, {Katie K.} and Allison Hubel",

note = "Funding Information: Acknowledgments This work was supported by the University of Minnesota Grant-in-aid Program, the National Blood Foundation and the National Institutes of Health (Grant No. R21EB004857). We would like to thank Brian Darr for his invaluable help and Dave Hultman, from the Research Shop at the Department of Electrical and Computer Engineering for helping with the design and fabrication of the microfluidic device. Anthony Cacace is acknowledged for helping with the exploded view of the channel.",

year = "2008",

doi = "10.1007/s10404-008-0265-9",

language = "English (US)",

volume = "5",

pages = "529--540",

journal = "Microfluidics and Nanofluidics",

issn = "1613-4982",

publisher = "Springer Verlag",

number = "4",

}

TY - JOUR

T1 - Experimental study of diffusion-based extraction from a cell suspension

AU - Mata, Clara

AU - Longmire, Ellen K.

AU - McKenna, David H.

AU - Glass, Katie K.

AU - Hubel, Allison

N1 - Funding Information: Acknowledgments This work was supported by the University of Minnesota Grant-in-aid Program, the National Blood Foundation and the National Institutes of Health (Grant No. R21EB004857). We would like to thank Brian Darr for his invaluable help and Dave Hultman, from the Research Shop at the Department of Electrical and Computer Engineering for helping with the design and fabrication of the microfluidic device. Anthony Cacace is acknowledged for helping with the exploded view of the channel.

PY - 2008

Y1 - 2008

N2 - A recently proposed application of microfluidics is the post-thaw processing of biological cells. Numerical simulations suggest that diffusion-based extraction of the cryoprotective agent dimethyl sulfoxide (DMSO) from blood cells is viable and more efficient than centrifugation, the conventional method of DMSO removal. In order to validate the theoretical model used in these simulations, a prototype was built and the flow of two parallel streams, a suspension of Jurkat cells containing DMSO and a wash stream that contained neither cells nor DMSO, was characterized experimentally. DMSO transport in a rectangular channel (depth 500 μm, width 25 mm and overall length 125 mm) was studied as a function of three dimensionless parameters: depth ratio of the streams, cell volume fraction in the cell solution, and the Peclet number (Pe ) based on channel depth, average flow rate and the diffusion coefficient for DMSO in water. In our studies, values of Pe ranged from O(103) to O(104). Laminar flow was ensured by keeping the Reynolds number between O(1) and O(10). Experimental results based on visual and quantitative data demonstrate conclusively that a microfluidic device can effectively remove DMSO from liquid and cell laden streams without compromising cell recovery. Also, flow conditions in the microfluidic device appear to have no adverse effect on cell viability at the outlet. Further, the results demonstrate that we can predict the amount of DMSO removed from a given device with the theoretical model mentioned previously.

AB - A recently proposed application of microfluidics is the post-thaw processing of biological cells. Numerical simulations suggest that diffusion-based extraction of the cryoprotective agent dimethyl sulfoxide (DMSO) from blood cells is viable and more efficient than centrifugation, the conventional method of DMSO removal. In order to validate the theoretical model used in these simulations, a prototype was built and the flow of two parallel streams, a suspension of Jurkat cells containing DMSO and a wash stream that contained neither cells nor DMSO, was characterized experimentally. DMSO transport in a rectangular channel (depth 500 μm, width 25 mm and overall length 125 mm) was studied as a function of three dimensionless parameters: depth ratio of the streams, cell volume fraction in the cell solution, and the Peclet number (Pe ) based on channel depth, average flow rate and the diffusion coefficient for DMSO in water. In our studies, values of Pe ranged from O(103) to O(104). Laminar flow was ensured by keeping the Reynolds number between O(1) and O(10). Experimental results based on visual and quantitative data demonstrate conclusively that a microfluidic device can effectively remove DMSO from liquid and cell laden streams without compromising cell recovery. Also, flow conditions in the microfluidic device appear to have no adverse effect on cell viability at the outlet. Further, the results demonstrate that we can predict the amount of DMSO removed from a given device with the theoretical model mentioned previously.

KW - Cell suspension

KW - Channel flow

KW - DMSO-extraction

KW - Diffusion

KW - Microfluidics

UR - http://www.scopus.com/inward/record.url?scp=50649115957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50649115957&partnerID=8YFLogxK

U2 - 10.1007/s10404-008-0265-9

DO - 10.1007/s10404-008-0265-9

M3 - Article

AN - SCOPUS:50649115957

SN - 1613-4982

VL - 5

SP - 529

EP - 540

JO - Microfluidics and Nanofluidics

JF - Microfluidics and Nanofluidics

IS - 4

ER -

Experimental study of diffusion-based extraction from a cell suspension

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this