Experimental mouse lethality of Escherichia coli isolates, in relation to accessory traits, phylogenetic group, and ecological source

James R. Johnson, Olivier Clermont, Megan Menard, Michael A. Kuskowski, Bertrand Picard, Erick Denamur

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

Background. Whether accessory traits, phylogenetic background, or ecological source best predicts extraintestinal virulence within Escherichia coli is undefined. Methods. A total of 90 E. coli strains (18 fecal isolates and 72 extraintestinal-infection isolates) were characterized for 55 accessory traits and phylogenetic group (A, B1, B2, or D). Bacterial traits and ecological source were compared with experimental mouse lethality. Results. Of the 90 strains, 41% were "killers" (i.e., killed ≥90% of mice). By univariate analysis, multiple group B2-associated traits (including malX [pathogenicity-island marker], pap [P fimbriae] elements, usp [uropathogenic-specific protein], and fyuA [yersiniabactin system]) were most closely associated with killer status, followed by group B2 (or non-group A) status and then by nonfecal origin. Stepwise multivariate analysis identified pap, malX, usp, fyuA, and B2 (all of which were positive predictors) and ireA (which was a negative predictor) as significant predictors of killer status. Killer strains segregated significantly from nonkiller strains, according to accessory-trait profiles. Factorial analysis of correspondence placed group B2 among the traits most closely associated with killer status, but not as the closest. Conclusions. Specific group B2-associated accessory traits are more potent predictors of experimental virulence among E. coli isolates than is either phylogenetic background or ecological source. Molecular typing can estimate an E. coli isolate's extraintestinal virulence potential, regardless of source.

Original languageEnglish (US)
Pages (from-to)1141-1150
Number of pages10
JournalJournal of Infectious Diseases
Volume194
Issue number8
DOIs
StatePublished - Oct 15 2006

Bibliographical note

Funding Information:
Financial support: Office of Research and Development, Medical Research Service, Department of Veterans Affairs (J.R.J.); Fondation pour la Recherche Médicale (E.D.).

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