Experimental Animal Models for Studying Antimicrobial Pharmacokinetics in Otitis Media

Daniel M. Canafax, Naobumi Nonomura, Gary R. Erdmann, Chap T Le, Steven S Juhn, G. Scott Giebink

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Antimicrobial treatment of otitis media, especially drug dosing considerations, is largely empiric, with few reported pharmacologic studies of drug distribution into the middle ear. A chinchilla animal model of serous and purulent otitis media has been used for some time to investigate mechanisms of disease pathogenesis. This model was adapted to investigate the penetration of amoxicillin, trimethoprim, and sulfamethoxazole into middle ear effusion. Purulent otitis media was produced by direct middle ear inoculation with type 7F Streptococcus pneumoniae. Serous otitis media was produced by eustachian tube obstruction using silastic sponge or Coeflex cement, but the Coeflex caused an undesirable local inflammatory response. The three antibiotics were administered to chinchillas with serous and purulent middle ear effusion. Plasma and ear fluid drug concentrations were measured by liquid chromatography and demonstrated the value of this model in assessing antibiotic penetration.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalPharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists
Volume6
Issue number4
DOIs
StatePublished - Jan 1 1989

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Otitis Media with Effusion
Pharmacokinetics
Otitis Media
Animals
Animal Models
Chinchilla
Suppurative Otitis Media
Middle Ear
Pharmaceutical Preparations
Anti-Bacterial Agents
Liquid chromatography
Amoxicillin
Sulfamethoxazole Drug Combination Trimethoprim
Eustachian Tube
Cements
Porifera
Streptococcus pneumoniae
Liquid Chromatography
Ear
Plasmas

Keywords

  • amoxicillin
  • otitis media
  • pharmacokinetics
  • sulfamethoxazole
  • trimethoprim

Cite this

Experimental Animal Models for Studying Antimicrobial Pharmacokinetics in Otitis Media. / Canafax, Daniel M.; Nonomura, Naobumi; Erdmann, Gary R.; Le, Chap T; Juhn, Steven S; Giebink, G. Scott.

In: Pharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists, Vol. 6, No. 4, 01.01.1989, p. 279-285.

Research output: Contribution to journalArticle

Canafax, Daniel M. ; Nonomura, Naobumi ; Erdmann, Gary R. ; Le, Chap T ; Juhn, Steven S ; Giebink, G. Scott. / Experimental Animal Models for Studying Antimicrobial Pharmacokinetics in Otitis Media. In: Pharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists. 1989 ; Vol. 6, No. 4. pp. 279-285.
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