Abstract
Antimicrobial treatment of otitis media, especially drug dosing considerations, is largely empiric, with few reported pharmacologic studies of drug distribution into the middle ear. A chinchilla animal model of serous and purulent otitis media has been used for some time to investigate mechanisms of disease pathogenesis. This model was adapted to investigate the penetration of amoxicillin, trimethoprim, and sulfamethoxazole into middle ear effusion. Purulent otitis media was produced by direct middle ear inoculation with type 7F Streptococcus pneumoniae. Serous otitis media was produced by eustachian tube obstruction using silastic sponge or Coeflex cement, but the Coeflex caused an undesirable local inflammatory response. The three antibiotics were administered to chinchillas with serous and purulent middle ear effusion. Plasma and ear fluid drug concentrations were measured by liquid chromatography and demonstrated the value of this model in assessing antibiotic penetration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 279-285 |
| Number of pages | 7 |
| Journal | Pharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 1 1989 |
Fingerprint
Keywords
- amoxicillin
- otitis media
- pharmacokinetics
- sulfamethoxazole
- trimethoprim
Cite this
Experimental Animal Models for Studying Antimicrobial Pharmacokinetics in Otitis Media. / Canafax, Daniel M.; Nonomura, Naobumi; Erdmann, Gary R.; Le, Chap T; Juhn, Steven S; Giebink, G. Scott.
In: Pharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists, Vol. 6, No. 4, 01.01.1989, p. 279-285.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Experimental Animal Models for Studying Antimicrobial Pharmacokinetics in Otitis Media
AU - Canafax, Daniel M.
AU - Nonomura, Naobumi
AU - Erdmann, Gary R.
AU - Le, Chap T
AU - Juhn, Steven S
AU - Giebink, G. Scott
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Antimicrobial treatment of otitis media, especially drug dosing considerations, is largely empiric, with few reported pharmacologic studies of drug distribution into the middle ear. A chinchilla animal model of serous and purulent otitis media has been used for some time to investigate mechanisms of disease pathogenesis. This model was adapted to investigate the penetration of amoxicillin, trimethoprim, and sulfamethoxazole into middle ear effusion. Purulent otitis media was produced by direct middle ear inoculation with type 7F Streptococcus pneumoniae. Serous otitis media was produced by eustachian tube obstruction using silastic sponge or Coeflex cement, but the Coeflex caused an undesirable local inflammatory response. The three antibiotics were administered to chinchillas with serous and purulent middle ear effusion. Plasma and ear fluid drug concentrations were measured by liquid chromatography and demonstrated the value of this model in assessing antibiotic penetration.
AB - Antimicrobial treatment of otitis media, especially drug dosing considerations, is largely empiric, with few reported pharmacologic studies of drug distribution into the middle ear. A chinchilla animal model of serous and purulent otitis media has been used for some time to investigate mechanisms of disease pathogenesis. This model was adapted to investigate the penetration of amoxicillin, trimethoprim, and sulfamethoxazole into middle ear effusion. Purulent otitis media was produced by direct middle ear inoculation with type 7F Streptococcus pneumoniae. Serous otitis media was produced by eustachian tube obstruction using silastic sponge or Coeflex cement, but the Coeflex caused an undesirable local inflammatory response. The three antibiotics were administered to chinchillas with serous and purulent middle ear effusion. Plasma and ear fluid drug concentrations were measured by liquid chromatography and demonstrated the value of this model in assessing antibiotic penetration.
KW - amoxicillin
KW - otitis media
KW - pharmacokinetics
KW - sulfamethoxazole
KW - trimethoprim
UR - http://www.scopus.com/inward/record.url?scp=0024372819&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024372819&partnerID=8YFLogxK
U2 - 10.1023/A:1015938205892
DO - 10.1023/A:1015938205892
M3 - Article
VL - 6
SP - 279
EP - 285
JO - Pharmaceutical Research
JF - Pharmaceutical Research
SN - 0724-8741
IS - 4
ER -