Abstract
The real-time polymerase chain reaction of the plant gene heat shock transcription factor was fully inhibited in the presence of a fullerene derivative C60(OH)20 at a concentration of 4 × 10-4mM. This inhibition was attributed to the interaction between the nanoparticle and Taq DNA polymerase in the reaction. Atomistic molecular dynamics simulations showed a clear tendency for hydrogen bonding between C 60(OH)20 and both the dNTPs and ssDNA components of the polymerase chain reaction. These studies facilitate our understanding of the fate of nanoparticles in biomolecular systems, a topic of tremendous importance for addressing the biological and environmental implications of nanomaterials.
Original language | English (US) |
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Article number | 415101 |
Journal | Nanotechnology |
Volume | 20 |
Issue number | 41 |
DOIs | |
State | Published - 2009 |
Externally published | Yes |