Expansion and hepatic differentiation of rat multipotent adult progenitor cells in microcarrier suspension culture

Y. Park, K. Subramanian, C. M. Verfaillie, W. S. Hu

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Many potential applications of stem cells require large quantities of cells, especially those involving large organs such as the liver. For such applications, a scalable reactor system is desirable to ensure a reliable supply of sufficient quantities of differentiation competent or differentiated cells. We employed a microcarrier culture system for the expansion of undifferentiated rat multipotent adult progenitor cells (rMAPC) as well as for directed differentiation of these cells to hepatocyte-like cells. During the 4-day expansion culture, cell concentration increased by 85-fold while expression level of pluripotency markers were maintained, as well as the MAPC differentiation potential. Directed differentiation into hepatocyte-like cells on the microcarriers themselves gave comparable results as observed with cells cultured in static cultures. The cells expressed several mature hepatocyte-lineage genes and asialoglycoprotein receptor-1 (ASGPR-1) surface protein, and secreted albumin and urea. Microcarrier culture thus offers the potential of large-scale expansion and differentiation of stem cells in a more controlled bioreactor environment.

Original languageEnglish (US)
Pages (from-to)131-139
Number of pages9
JournalJournal of Biotechnology
Volume150
Issue number1
DOIs
StatePublished - Oct 1 2010

Keywords

  • Bioreactors
  • Hepatic differentiation
  • MAPC
  • Microcarrier

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