Exogenic glucose as an electron donor for algal hydrogenases to promote hydrogen photoproduction by Chlorella pyrenoidosa

Jun Zhi Liu, Ya Ming Ge, Jing Ya Sun, Paul Chen, Min Addy, Shu Hao Huo, Kun Li, Peng Fei Cheng, Roger Ruan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In this work, glucose addition (0.7 g l−1) almost doubled hydrogen yield of Chlorella pyrenoidosa (121.1 ml l−1 vs 65.5 ml l−1), with a positive correlation between hydrogen production and glucose consumption (−0.977, P < 0.01). When the electrons transport from water photolysis to algal hydrogenase was inhibited, the hydrogen productivity declined by 21.1%; whereas it dramatically decreased by 70.9% when the algal nicotinamide adeninedinucleotide dehydrogenase (NADH) was inhibited. Therefore, in the presence of glucose, the electrons for algae based hydrogen production would be mainly from glucose glycolysis rather than water photolysis. Further deuterated-glucose trial indicated that the glucose might serve as an electron donor for algal hydrogenases. Finally, a tentative electron transport route from glucose to algal hydrogenase was proposed, hoping to provide more scientific direction for further algae-based hydrogen production improvement.

Original languageEnglish (US)
Article number121762
JournalBioresource Technology
Volume289
DOIs
StatePublished - Oct 2019

Bibliographical note

Funding Information:
This research was financially supported by Zhejiang Provincial Natural Science Foundation of China ( LQ17C030001 ), Scientific Research Fund of Zhejiang Provincial Education Department ( Y201840278 ) and China Scholarship Council ( CSC ). We gratefully acknowledge all the reviewers for their great contribution to the improvement of this article.

Funding Information:
This research was financially supported by Zhejiang Provincial Natural Science Foundation of China (LQ17C030001), Scientific Research Fund of Zhejiang Provincial Education Department (Y201840278) and China Scholarship Council (CSC). We gratefully acknowledge all the reviewers for their great contribution to the improvement of this article.

Publisher Copyright:
© 2019 Elsevier Ltd

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • Algal H production
  • Deuterium
  • Glucose
  • NADH
  • Sulfur-deprivation

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