Exogenic glucose as an electron donor for algal hydrogenases to promote hydrogen photoproduction by Chlorella pyrenoidosa

Jun Zhi Liu, Ya Ming Ge, Jing Ya Sun, Paul L Chen, Min M Addy, Shu Hao Huo, Kun Li, Peng Fei Cheng, R. R Ruan

Research output: Contribution to journalArticle

Abstract

In this work, glucose addition (0.7 g l−1) almost doubled hydrogen yield of Chlorella pyrenoidosa (121.1 ml l−1 vs 65.5 ml l−1), with a positive correlation between hydrogen production and glucose consumption (−0.977, P < 0.01). When the electrons transport from water photolysis to algal hydrogenase was inhibited, the hydrogen productivity declined by 21.1%; whereas it dramatically decreased by 70.9% when the algal nicotinamide adeninedinucleotide dehydrogenase (NADH) was inhibited. Therefore, in the presence of glucose, the electrons for algae based hydrogen production would be mainly from glucose glycolysis rather than water photolysis. Further deuterated-glucose trial indicated that the glucose might serve as an electron donor for algal hydrogenases. Finally, a tentative electron transport route from glucose to algal hydrogenase was proposed, hoping to provide more scientific direction for further algae-based hydrogen production improvement.

Original languageEnglish (US)
Article number121762
JournalBioresource Technology
Volume289
DOIs
StatePublished - Oct 1 2019

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Hydrogenase
Glucose
Hydrogen
glucose
hydrogen
electron
Electrons
Hydrogen production
Photolysis
Algae
photolysis
alga
Niacinamide
Water
Oxidoreductases
Productivity
water
productivity

Keywords

  • Algal H production
  • Deuterium
  • Glucose
  • NADH
  • Sulfur-deprivation

Cite this

Exogenic glucose as an electron donor for algal hydrogenases to promote hydrogen photoproduction by Chlorella pyrenoidosa. / Liu, Jun Zhi; Ge, Ya Ming; Sun, Jing Ya; Chen, Paul L; Addy, Min M; Huo, Shu Hao; Li, Kun; Cheng, Peng Fei; Ruan, R. R.

In: Bioresource Technology, Vol. 289, 121762, 01.10.2019.

Research output: Contribution to journalArticle

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AU - Liu, Jun Zhi

AU - Ge, Ya Ming

AU - Sun, Jing Ya

AU - Chen, Paul L

AU - Addy, Min M

AU - Huo, Shu Hao

AU - Li, Kun

AU - Cheng, Peng Fei

AU - Ruan, R. R

PY - 2019/10/1

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N2 - In this work, glucose addition (0.7 g l−1) almost doubled hydrogen yield of Chlorella pyrenoidosa (121.1 ml l−1 vs 65.5 ml l−1), with a positive correlation between hydrogen production and glucose consumption (−0.977, P < 0.01). When the electrons transport from water photolysis to algal hydrogenase was inhibited, the hydrogen productivity declined by 21.1%; whereas it dramatically decreased by 70.9% when the algal nicotinamide adeninedinucleotide dehydrogenase (NADH) was inhibited. Therefore, in the presence of glucose, the electrons for algae based hydrogen production would be mainly from glucose glycolysis rather than water photolysis. Further deuterated-glucose trial indicated that the glucose might serve as an electron donor for algal hydrogenases. Finally, a tentative electron transport route from glucose to algal hydrogenase was proposed, hoping to provide more scientific direction for further algae-based hydrogen production improvement.

AB - In this work, glucose addition (0.7 g l−1) almost doubled hydrogen yield of Chlorella pyrenoidosa (121.1 ml l−1 vs 65.5 ml l−1), with a positive correlation between hydrogen production and glucose consumption (−0.977, P < 0.01). When the electrons transport from water photolysis to algal hydrogenase was inhibited, the hydrogen productivity declined by 21.1%; whereas it dramatically decreased by 70.9% when the algal nicotinamide adeninedinucleotide dehydrogenase (NADH) was inhibited. Therefore, in the presence of glucose, the electrons for algae based hydrogen production would be mainly from glucose glycolysis rather than water photolysis. Further deuterated-glucose trial indicated that the glucose might serve as an electron donor for algal hydrogenases. Finally, a tentative electron transport route from glucose to algal hydrogenase was proposed, hoping to provide more scientific direction for further algae-based hydrogen production improvement.

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KW - Sulfur-deprivation

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