Exhaled breath condensate collection for nitrite dosage. A safe and low cost adaptation

Graziela Saraiva Reis, Viviane Dos Santos Augusto, Maria Eliza Jordani De Souza, Caroline Floreoto Baldo, Alfredo José Rodrigues, Paulo Roberto Barbosa Evora

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Purpose: Standardization of a simple and low cost technique of exhaled breath condensate (EBC) collection to measure nitrite. Methods: Two devices were mounted in polystyrene boxes filled either with crushed ice/salt crystals or dry ice/crushed ice. Blood samples were stored at -70° C for posterior nitrite dosages by chemiluminescence and the Griess reaction. Results: a) The use of crushed ice/dry ice or salt revealed sufficient EBC room air collection, but was not efficient for patients under ventilation support; b) the method using crushed ice/salt collected greater EBC volumes, but the nitrite concentrations were not proportional to the volume collected; c) The EBC nitrite values were higher in the surgical group using both methods; d) In the surgical group the nasal clip use diminished the EBC nitrite concentrations in both methods. Conclusions: The exhaled breath condensate (EBC) methodology collection was efficient on room air breathing. Either cooling methods provided successful EBC collections showing that it is possible to diminish costs, and, amongst the two used methods, the one using crushed ice/salt crystals revealed better efficiency compared to the dry ice method.

Original languageEnglish (US)
Pages (from-to)206-213
Number of pages8
JournalActa Cirurgica Brasileira
Issue number2
StatePublished - Mar 2010


  • Extracorporeal circulation
  • Nitrites
  • Thoracic surgery


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