Exercise attenuates low back pain and alters epigenetic regulation in intervertebral discs in a mouse model

Yuya Kawarai, Seon Ho Jang, Seunghwan Lee, Magali Millecamps, Hyung Mo Kang, Stephanie Gregoire, Miyako Suzuki-Narita, Seiji Ohtori, Laura S. Stone

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND CONTEXT: Chronic low back pain (LBP) is a multifactorial disorder with complex underlying mechanisms, including associations with intervertebral disc (IVD) degeneration in some individuals. It has been demonstrated that epigenetic processes are involved in the pathology of IVD degeneration. Epigenetics refers to several mechanisms, including DNA methylation, that have the ability to change gene expression without inducing any change in the underlying DNA sequence. DNA methylation can alter the entire state of a tissue for an extended period of time and thus could potentially be harnessed for long-term pain relief. Lifestyle factors, such as physical activity, have a strong influence on epigenetic regulation. Exercise is a commonly prescribed treatment for chronic LBP, and sex-specific epigenetic adaptations in response to endurance exercise have been reported. However, whether exercise interventions that attenuate LBP are associated with epigenetic alterations in degenerating IVDs has not been evaluated.

PURPOSE: We hypothesize that the therapeutic efficacy of physical activity is mediated, at least in part, at the epigenetic level. The purpose of this study was to use the SPARC-null mouse model of LBP associated with IVD degeneration to clarify (1) if IVD degeneration is associated with altered expression of epigenetic regulatory genes in the IVDs, (2) if epigenetic regulatory machinery is sensitive to therapeutic environmental intervention, and (3) if there are sex-specific differences in (1) and/or (2).

STUDY DESIGN: Eight-month-old male and female SPARC-null and age-matched control (WT) mice (n=108) were assigned to exercise (n=56) or sedentary (n=52) groups. Deletion of SPARC is associated with progressive IVD degeneration and behavioral signs of LBP. The exercise group received a circular plastic home cage running wheel on which they could run freely. The sedentary group received an identical wheel secured in place to prevent rotation. After 6 months, the results obtained in each group were compared.

METHODS: After 6 months of exercise, LBP-related behavioral indices were determined, and global DNA methylation (5-methylcytosine) and epigenetic regulatory gene mRNA expression in IVDs were assessed. This project was supported by the Canadian Institutes for Health Research. The authors have no conflicts of interest.

RESULTS: Lumbar IVDs from WT sedentary and SPARC-null sedentary mice had similar levels of global DNA methylation (%5-mC) and comparable mRNA expression of epigenetic regulatory genes (Dnmt1,3a,b, Mecp2, Mbd2a,b, Tet1-3) in both sexes. Exercise attenuated LBP-related behaviors, decreased global DNA methylation in both WT (p<.05) and SPARC-null mice (p<.01) and reduced mRNA expression of Mecp2 in SPARC-null mice (p<.05). Sex-specific effects of exercise on expression of mRNA were also observed.

CONCLUSIONS: Exercise alleviates LBP in a mouse model. This may be mediated, in part, by changes in the epigenetic regulatory machinery in degenerating IVDs. Epigenetic alterations due to a lifestyle change could have a long-lasting therapeutic impact by changing tissue homeostasis in IVDs.

CLINICAL SIGNIFICANCE: This study confirmed the therapeutic benefits of exercise on LBP and suggests that exercise results in sex-specific alterations in epigenetic regulation in IVDs. Elucidating the effects of exercise on epigenetic regulation may enable the discovery of novel gene targets or new strategies to improve the treatment of chronic LBP.

Original languageEnglish (US)
Pages (from-to)1938-1949
Number of pages12
JournalSpine Journal
Volume21
Issue number11
Early online dateJun 9 2021
DOIs
StatePublished - Nov 2021

Bibliographical note

Funding Information:
We thank the staff of McGill University's Comparative Medicine and Animal Resources Center, the Alan Edwards Centre for Research on Pain, and Dr. Moshe Syzf's lab for their support. This work was supported by the Canadian Institutes for Health Research Projects Grants MOP‐142291 to LSS and MM and PJT-148636 to LSS. YK was supported by the Uehara Memorial Foundation Overseas postdoctoral fellowships and postdoctoral training grant from the Fonds de Recherche du Québec Santé (No dossier 284676) . SL was supported by the Catherine Bushnell postdoctoral fellowship from the Louise and Alan Edwards Foundation .

Funding Information:
We thank the staff of McGill University's Comparative Medicine and Animal Resources Center, the Alan Edwards Centre for Research on Pain, and Dr. Moshe Syzf's lab for their support. This work was supported by the Canadian Institutes for Health Research Projects Grants MOP?142291 to LSS and MM and PJT-148636 to LSS. YK was supported by the Uehara Memorial Foundation Overseas postdoctoral fellowships and postdoctoral training grant from the Fonds de Recherche du Qu?bec Sant? (No dossier 284676). SL was supported by the Catherine Bushnell postdoctoral fellowship from the Louise and Alan Edwards Foundation.

Publisher Copyright:
© 2021 The Authors

Keywords

  • Chronic pain
  • DNA methylation
  • Epigenetics
  • SPARC-null
  • Sex differences
  • Voluntary running

PubMed: MeSH publication types

  • Journal Article

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