Examining the causes and consequences of context-specific differential DNA methylation in maize

Qing Li, Jawon Song, Patrick T. West, Greg Zynda, Steven R. Eichten, Matthew W. Vaughn, Nathan M. Springer

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

DNA methylation is a stable modification of chromatin that can contribute to epigenetic variation through the regulation of genes or transposons. Profiling of DNA methylation in five maize (Zea mays) inbred lines found that while DNA methylation levels for more than 99% of the analyzed genomic regions are similar, there are still 5,000 to 20,000 context-specific differentially methylated regions (DMRs) between any two genotypes. The analysis of identical-by-state genomic regions that have limited genetic variation provided evidence that DMRs can occur without local sequence variation, but they are less common than in regions with genetic variation. Characterization of the sequence specificity of DMRs, location of DMRs relative to genes and transposons, and patterns of DNA methylation in regions flanking DMRs reveals a distinct subset of DMRs. Transcriptome profiling of the same tissue revealed that only approximately 20% of genes with qualitative (on-off) differences in gene expression are associated with DMRs, and there is little evidence for association of DMRs with genes that show quantitative differences in gene expression. We also identify a set of genes that may represent cryptic information that is silenced by DNA methylation in the reference B73 genome. Many of these genes exhibit natural variation in other genotypes, suggesting the potential for selection to act upon existing epigenetic natural variation. This study provides insights into the origin and influences of DMRs in a crop species with a complex genome organization.

Original languageEnglish (US)
Pages (from-to)1262-1274
Number of pages13
JournalPlant physiology
Volume168
Issue number4
DOIs
StatePublished - Aug 1 2015

Bibliographical note

Publisher Copyright:
© 2015 American Society of Plant Biologists. All rights reserved.

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