Examination of CK2α and NF-κB p65 expression in human benign prostatic hyperplasia and prostate cancer tissues

Fatima Qaiser, Janeen H. Trembley, Sarah Sadiq, Iqbal Muhammad, Rubina Younis, Shoaib Naiyar Hashmi, Badar Murtaza, Thomas S. Rector, Abdul Khaliq Naveed, Khalil Ahmed

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Protein kinase CK2 plays a critical role in cell growth, proliferation, and suppression of cell death. CK2 is overexpressed, especially in the nuclear compartment, in the majority of cancers, including prostate cancer (PCa). CK2-mediated activation of transcription factor nuclear factor kappa B (NF-κB) p65 is a key step in cellular proliferation, resulting in translocation of NF-κB p65 from the cytoplasm to the nucleus. As CK2 expression and activity are also elevated in benign prostatic hyperplasia (BPH), we sought to increase the knowledge of CK2 function in benign and malignant prostate by examination of the relationships between nuclear CK2 and nuclear NF-κB p65 protein expression. The expression level and localization of CK2α and NF-κB p65 proteins in PCa and BPH tissue specimens was determined. Nuclear CK2α and NF-κB p65 protein levels are significantly higher in PCa compared with BPH, and these proteins are positively correlated with each other in both diseases. Nuclear NF-κB p65 levels correlated with Ki-67 or with cytoplasmic NF-κB p65 expression in BPH, but not in PCa. The findings provide information that combined analysis of CK2α and NF-κB p65 expression in prostate specimens relates to the disease status. Increased nuclear NF-κB p65 expression levels in PCa specifically related to nuclear CK2α levels, indicating a possible CK2-dependent relationship in malignancy. In contrast, nuclear NF-κB p65 protein levels related to both Ki-67 and cytoplasmic NF-κB p65 levels exclusively in BPH, suggesting a potential separate impact for NF-κB p65 function in proliferation for benign disease as opposed to malignant disease.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalMolecular and cellular biochemistry
Issue number1-2
StatePublished - Sep 1 2016

Bibliographical note

Funding Information:
This work was supported by United States Department of Veterans Affairs Merit Review Program Grant numbers I01BX001731 and I01BX003282, and by National Cancer Institute Grant number R01CA150182 (KA). National University of Sciences and Technology provided financial support under the Mega S&T scholarship fund for this work (FQ and SS). Armed Forces Institute of Pathology and Armed Forces Institute of Urology, Rawalpindi, provided technical assistance in the conduct of this work. The results shown here are in part based upon data generated by the TCGA Research Network: http://cancergenome.nih.gov/ .

Publisher Copyright:
© 2016, Springer Science+Business Media New York (outside the USA).


  • BPH
  • Biomarker
  • CK2
  • Ki-67
  • Nuclear factor kappa B
  • Prostate cancer
  • RELA


Dive into the research topics of 'Examination of CK2α and NF-κB p65 expression in human benign prostatic hyperplasia and prostate cancer tissues'. Together they form a unique fingerprint.

Cite this