Evolutionary meta-analysis of solanaceous resistance gene and solanum resistance gene analog sequences and a practical framework for cross-species comparisons

Edmund A. Quirin, Harpartap Mann, Rachel S. Meyer, Alessandra Traini, Maria Luisa Chiusano, Amy Litt, James M. Bradeen

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Cross-species comparative genomics approaches have been employed to map and clone many important disease resistance (R) genes from Solanum species - especially wild relatives of potato and tomato. These efforts will increase with the recent release of potato genome sequence and the impending release of tomato genome sequence. Most R genes belong to the prominent nucleotide binding site-leucine rich repeat (NBS-LRR) class and conserved NBS-LRR protein motifs enable survey of the R gene space of a plant genome by generation of resistance gene analogs (RGA), polymerase chain reaction fragments derived from R genes. We generated a collection of 97 RGA from the disease-resistant wild potato S. bulbocastanum, complementing smaller collections from other Solanum species. To further comparative genomics approaches, we combined all known Solanum RGA and cloned solanaceous NBS-LRR gene sequences, nearly 800 sequences in total, into a single meta-analysis. We defined R gene diversity bins that reflect both evolutionary relationships and DNA cross-hybridization results. The resulting framework is amendable and expandable, providing the research community with a common vocabulary for present and future study of R gene lineages. Through a series of sequence and hybridization experiments, we demonstrate that all tested R gene lineages are of ancient origin, are shared between Solanum species, and can be successfully accessed via comparative genomics approaches.

Original languageEnglish (US)
Pages (from-to)603-612
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume25
Issue number5
DOIs
StatePublished - May 2012

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