Evolutionary conservation of MyoD function and differential utilization of E proteins

Jian Min Zhang, Lihsia Chen, Michael Krause, Andrew Fire, Bruce M. Paterson

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The formation of striated muscle in both vertebrates and invertebrates involves the activity of the MyoD family of basic-helix-loop-helix (bHLH) transcription factors. The high degree of evolutionary conservation of MyoD- related proteins, both in the sequence of their bHLH domains and in their general developmental expression patterns, suggests that these factors are also conserved at the level of function. We have addressed this directly using MyoD and E protein factors from vertebrates, Drosophila, and Caenorhabditis elegans. Various MyoD and E factor combinations were tested for their ability to interact in vitro and to function in vivo in the myogenic conversion of 10T 1/2 mouse fibroblasts. We found that the ability of different homo- and heterodimers to bind DNA in vitro was an accurate measure of biological activity in vivo. A second assessment of conserved function comes from the ability of these factors to rescue a C. elegans hlh-1 (CeMyoD) null mutation. We found that both Drosophila and chicken MyoD-related factors were able to rescue a C. elegans CeMyoD loss-of-function mutation. These results demonstrate a remarkable degree of functional conservation of these myogenic factors despite differences in E-protein interactions.

Original languageEnglish (US)
Pages (from-to)465-472
Number of pages8
JournalDevelopmental Biology
Issue number2
StatePublished - Apr 15 1999


  • C. elegans
  • Drosophila
  • E proteins
  • MyoD
  • Myogenesis


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