Evolution and expression of Translationally Controlled Tumour Protein (TCTP) of fish

Thayanithy Venugopal

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Translationally Controlled Tumour Protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans. In order to better understand its functions in non-mammalians, cDNA encoding full-length TCTP has been isolated and characterized from a teleost fish, Labeo rohita (rohu). Encoded by a 1043 nucleotide mRNA, rohu TCTP consists of 171 amino acids and is expressed in all organs, except in brain. Secondary structure of fish TCTP mRNAs shows that they could be potential substrates for RNA specific protein kinase PKR. The three-dimensional structure of rohu TCTP has been determined, as the first metazoan model of this protein. The conservational and phylogenic clustering of plant and animal TCTP sequences is consistent with the eukaryotic classification, and is suggestive of early origin for the TCTP ortholog in eukaryote evolution, as early as 1.0 × 109 years ago. Despite significant conservation, meticulous sequence analysis reveals striking differences that suggest possible new functions for TCTP.

Original languageEnglish (US)
Pages (from-to)8-17
Number of pages10
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume142
Issue number1
DOIs
StatePublished - Sep 2005

Keywords

  • Evolution
  • Gene expression
  • Labeo rohita
  • Ortholog
  • Phylogeny
  • Protein kinase PKR
  • RNA secondary structure
  • Sequence homology
  • Three-dimensional structure
  • Translationallly controlled tumour protein

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