Evidence that pseudorenin activity in bovine spleen is due to cathepsin D

Rodney L. Johnson; Alan M. Poisner

doi:10.1016/0006-2952(77)90285-4

Evidence that pseudorenin activity in bovine spleen is due to cathepsin D

Rodney L. Johnson
, Alan M. Poisner

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Pseudorenin and cathepsin D activity from bovine spleen were found to behave identically on DEAE-cellulose, Sephadex G-100, and concanavalin A-agarose chromatography. The molecular weight of pseudorenin and of cathepsin D was estimated to be 50,000. The binding of the enzymatic activity to concanavalin A-agarose and elution with α-methyl-d-mannoside indicates that pseudorenin (cathepsin D) is a glycoprotein. It is suggested that pseudorenin activity in the spleen is due to cathepsin D.

Original language	English (US)
Pages (from-to)	2237-2240
Number of pages	4
Journal	Biochemical Pharmacology
Volume	26
Issue number	23
DOIs	https://doi.org/10.1016/0006-2952(77)90285-4
State	Published - Dec 1 1977

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title = "Evidence that pseudorenin activity in bovine spleen is due to cathepsin D",

abstract = "Pseudorenin and cathepsin D activity from bovine spleen were found to behave identically on DEAE-cellulose, Sephadex G-100, and concanavalin A-agarose chromatography. The molecular weight of pseudorenin and of cathepsin D was estimated to be 50,000. The binding of the enzymatic activity to concanavalin A-agarose and elution with α-methyl-d-mannoside indicates that pseudorenin (cathepsin D) is a glycoprotein. It is suggested that pseudorenin activity in the spleen is due to cathepsin D.",

author = "Johnson, \{Rodney L.\} and Poisner, \{Alan M.\}",

year = "1977",

month = dec,

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doi = "10.1016/0006-2952(77)90285-4",

language = "English (US)",

volume = "26",

pages = "2237--2240",

journal = "Biochemical Pharmacology",

issn = "0006-2952",

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TY - JOUR

T1 - Evidence that pseudorenin activity in bovine spleen is due to cathepsin D

AU - Johnson, Rodney L.

AU - Poisner, Alan M.

PY - 1977/12/1

Y1 - 1977/12/1

N2 - Pseudorenin and cathepsin D activity from bovine spleen were found to behave identically on DEAE-cellulose, Sephadex G-100, and concanavalin A-agarose chromatography. The molecular weight of pseudorenin and of cathepsin D was estimated to be 50,000. The binding of the enzymatic activity to concanavalin A-agarose and elution with α-methyl-d-mannoside indicates that pseudorenin (cathepsin D) is a glycoprotein. It is suggested that pseudorenin activity in the spleen is due to cathepsin D.

AB - Pseudorenin and cathepsin D activity from bovine spleen were found to behave identically on DEAE-cellulose, Sephadex G-100, and concanavalin A-agarose chromatography. The molecular weight of pseudorenin and of cathepsin D was estimated to be 50,000. The binding of the enzymatic activity to concanavalin A-agarose and elution with α-methyl-d-mannoside indicates that pseudorenin (cathepsin D) is a glycoprotein. It is suggested that pseudorenin activity in the spleen is due to cathepsin D.

UR - https://www.scopus.com/pages/publications/0017574914

UR - https://www.scopus.com/pages/publications/0017574914#tab=citedBy

U2 - 10.1016/0006-2952(77)90285-4

DO - 10.1016/0006-2952(77)90285-4

M3 - Article

C2 - 588308

AN - SCOPUS:0017574914

SN - 0006-2952

VL - 26

SP - 2237

EP - 2240

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 23

ER -

Evidence that pseudorenin activity in bovine spleen is due to cathepsin D

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