Evidence that petromyzontid lampreys employ a common migratory pheromone that is partially comprised of bile acids

Jared M. Fine, Lance A. Vrieze, Peter W. Sorensen

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

This study examined whether the larval pheromone employed by adult sea lamprey (Petromyzon marinus) to locate spawning streams and known to be at least partially comprised of bile acids is also employed by other lamprey species. Both production and release of lamprey-specific bile acids, and sensitivity to them were examined in a wide variety of species. High pressure liquid chromatography and electrospray ionization/mass spectrometry (ESI-MS) found gallbladders from 10 species of European and North American lamprey to contain large quantities of petromyzonol sulfate (PS) together with much smaller quantities of allocholic acid (ACA) and petromyzonol (P). Evaluation of holding waters from three of these species using ESI-MS found all to contain large quantities of PS and lesser quantities of ACA in similar ratios. Electro-olfactogram recording from the olfactory systems of three parasitic lamprey species found all to detect PS and ACA with high sensitivity. Behavioral studies using migratory adult sea lamprey found them to be attracted to the odors of heterospecific larvae as well as conspecific larvae, both of which contained similar amounts of PS and ACA. Finally, adult silver lampreys (Ichthyomyzon unicuspis) were also found to be attracted to the odor of larval sea lamprey. Together, these results demonstrate that PS and ACA are commonly produced and released by larval petromyzontid lampreys and likely used as part of a common evolutionarily conserved pheromone. This scenario is reasonable because lampreys share similar larval and spawning habitat requirements, and their larvae derive no apparent benefit from producing compounds that serve as an attractant for adults.

Original languageEnglish (US)
Pages (from-to)2091-2110
Number of pages20
JournalJournal of Chemical Ecology
Volume30
Issue number11
DOIs
StatePublished - Nov 2004

Bibliographical note

Funding Information:
Acknowledgments—Thanks to the staff of the Hammond Bay Biological Station (U.S. Geological Survey) who provided assistance and access to their facilities. The authors thank Judith Olson for performing some of the HPLC analyses of gallbladders, and Cheryl Murphy and Rickard Bjerselius for helping with EOG recording. Ludington Biological Station staff collected the L. appendix and I. fossor larvae, while Dr Kimmo Aronsuu collected L. fluviatilis and L. planeri larvae. The Marquette Biological Station (U.S. Fish and Wildlife Service), the Sea Lamprey Control Centre (Department of Fisheries and Oceans, Sault Ste. Marie, Canada), Brad Young (Michigan State University), and David Close (Confederated Tribes of the Umatilla Indian Reservation, Oregon) supplied migratory animals and some larvae. Thanks also to Thomas Krick and Leeann Higgins of the mass spectrometry consortium at the University of Minnesota for their advice and use of their facilities. Lefric Enwall and Daniel Kurkiewicz assisted with behavior experiments. Andrew Simons and Beth Anderson read early versions of this paper. Funding was provided by the Great Lakes Fishery Commission.

Keywords

  • Allocholic acid
  • Evolution
  • Lamprey
  • Migration
  • Petromyzonol
  • Petromyzonol sulfate
  • Petromyzontidae
  • Pheromone
  • Species-specificity

Fingerprint

Dive into the research topics of 'Evidence that petromyzontid lampreys employ a common migratory pheromone that is partially comprised of bile acids'. Together they form a unique fingerprint.

Cite this