Evidence that an Unnamed Isometric Virus Associated with Potato Rugose Disease in Peru Is a New Species of Genus Torradovirus

Robert Alvarez-Quinto, Melody Amao, Giovanna Muller, Segundo Fuentes, Samuel Grinstead, Irazema Fuentes-Bueno, Annelien Roenhorst, Marcel Westenberg, Marleen Botermans, Jan Kreuze, Dimitre Mollov

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A previously uncharacterized torradovirus species infecting potatoes was detected by high-throughput sequencing from field samples from Peru and in customs intercepts in potato tubers that originated from South America in the United States of America and the Netherlands. This new potato torradovirus showed high nucleotide sequence identity to an unidentified isometric virus (SB26/29), which was associated with a disease named potato rugose stunting in southern Peru characterized over two decades ago. Thus, this virus is tentatively named potato rugose stunting virus (PotRSV). The genome of PotRSV isolates sequenced in this study were composed of two polyadenylated RNA segments. RNA1 ranges from 7,086 to 7,089 nt and RNA2 from 5,228 to 5,230 nt. RNA1 encodes a polyprotein containing the replication block (helicase-protease-polymerase), whereas RNA2 encodes a polyprotein cleaved into a movement protein and the three capsid proteins (CPs). Pairwise comparison among PotRSV isolates revealed amino acid identity values greater than 86% in the protease-polymerase (Pro-Pol) region and greater than 82% for the combined CPs. The closest torradovirus species, squash chlorotic leaf spot virus, shares amino acid identities of ∼58 and ∼41% in the Pro-Pol and the combined CPs, respectively.

Original languageEnglish (US)
Pages (from-to)1716-1728
Number of pages13
JournalPhytopathology
Volume113
Issue number9
DOIs
StatePublished - Sep 2023
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2023 The Author(s).

Keywords

  • HTS
  • characterization
  • potato
  • potato rugose stunting virus
  • torradovirus
  • virus discovery
  • virus disease

PubMed: MeSH publication types

  • Journal Article

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