TY - JOUR
T1 - Evidence of human immunodeficiency virus type 1 infection of nestin-positive neural progenitors in archival pediatric brain tissue
AU - Schwartz, Lynnae
AU - Civitello, Lucy
AU - Dunn-Pirio, Anastasie
AU - Ryschkewitsch, Samantha
AU - Berry, Elizabeth
AU - Cavert, Winston
AU - Kinzel, Nikilyn
AU - Lawrence, Diane M.P.
AU - Hazra, Rohan
AU - Major, Eugene O.
PY - 2007/5
Y1 - 2007/5
N2 - Human immunodeficiency virus type 1 (HIV-1) central nervous system (CNS) infection in children is associated with impaired brain growth and neurodevelopmental delays. Neural progenitors are critical for neurogenesis. Human multipotential neural progenitors grown in culture are permissive for HIV-1 infection, but it is not known if infection of these cells occurs in vivo. Brain tissue from pre-highly active antiretroviral therapy (HAART) era pediatric acquired immunodeficiency syndrome (AIDS) patients was examined for evidence of HIV-1 infection of nestin-positive neural progenitors by in situ hybridization; or after laser microdissection harvest, DNA extraction, and polymerase chain reaction (PCR). HIV-1 or viral DNA was identified in nestin-positive cells in four of seven HIV-1-infected children, suggesting in vivo infection of neural progenitors.
AB - Human immunodeficiency virus type 1 (HIV-1) central nervous system (CNS) infection in children is associated with impaired brain growth and neurodevelopmental delays. Neural progenitors are critical for neurogenesis. Human multipotential neural progenitors grown in culture are permissive for HIV-1 infection, but it is not known if infection of these cells occurs in vivo. Brain tissue from pre-highly active antiretroviral therapy (HAART) era pediatric acquired immunodeficiency syndrome (AIDS) patients was examined for evidence of HIV-1 infection of nestin-positive neural progenitors by in situ hybridization; or after laser microdissection harvest, DNA extraction, and polymerase chain reaction (PCR). HIV-1 or viral DNA was identified in nestin-positive cells in four of seven HIV-1-infected children, suggesting in vivo infection of neural progenitors.
KW - Gag
KW - Laser microdissection
KW - Nestin
KW - Neural progenitors
KW - Pediatric neuroAIDS
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U2 - 10.1080/13550280701344975
DO - 10.1080/13550280701344975
M3 - Article
C2 - 17613718
AN - SCOPUS:34447299925
SN - 1355-0284
VL - 13
SP - 274
EP - 283
JO - Journal of neurovirology
JF - Journal of neurovirology
IS - 3
ER -