An adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 220.127.116.11] preparation that is not stimulated by NaF,5'-guanylyl imidodiphosphate, or Ca2+-calmodulin has been isolated from bovine cerebral cortex by Affi-Gel Blue chromatography and calmodulin-Sepharose chromatography. Sensitivity to these effectors was restored by incubation of the adenylate cyclase preparation with detergent-solubilized protein from bovine cerebral cortex. Reconstitution of Ca2+ calmodulin activation required the presence of 5'-guanylyl imidodiphosphate. The factor required for restoration of Ca2+ calmodulin stimulation was sensitive to heat, trypsin digestion, and N-ethylmaleimide. These observations suggested that this adenylate cyclase activity requires the presence of one or more guanyl nucleotide binding subunits for calmodulin sensitivity.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1979|