Evidence for Phenotypic Plasticity in Aggressive Triple-Negative Breast Cancer: Human Biology Is Recapitulated by a Novel Model System

Julie H Ostrander, Nicholas C. D'Amato, Michelle L. Bowie, Christopher Sistrunk, Alexander Borowsky, Robert D. Cardiff, Katie Bell, Lawrence J.T. Young, Karl Simin, Robin E. Bachelder, Jeff Delrow, Alyssa Dawson, Lisa D. Yee, Krzysztof Mrózek, Timothy M. Clay, Takuya Osada, Victoria L. Seewaldt

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Breast cancers with a basal-like gene signature are primarily triple-negative, frequently metastatic, and carry a poor prognosis. Basal-like breast cancers are enriched for markers of breast cancer stem cells as well as markers of epithelial-mesenchymal transition (EMT). While EMT is generally thought to be important in the process of metastasis, in vivo evidence of EMT in human disease remains rare. Here we report a novel model of human triple-negative breast cancer, the DKAT cell line, which was isolated from an aggressive, treatment-resistant triple-negative breast cancer that demonstrated morphological and biochemical evidence suggestive of phenotypic plasticity in the patient. The DKAT cell line displays a basal-like phenotype in vitro when cultured in serum-free media, and undergoes phenotypic changes consistent with EMT/MET in response to serum-containing media, a unique property among the breast cancer cell lines we tested. This EMT is marked by increased expression of the transcription factor Zeb1, and Zeb1 is required for the enhanced migratory ability of DKAT cells in the mesenchymal state. DKAT cells also express progenitor-cell markers, and single DKAT cells are able to generate tumorspheres containing both epithelial and mesenchymal cell types. In vivo, as few as ten DKAT cells are capable of forming xenograft tumors which display a range of epithelial and mesenchymal phenotypes. The DKAT model provides a novel model to study the molecular mechanisms regulating phenotypic plasticity and the aggressive biology of triple-negative breast cancers.

Original languageEnglish (US)
Article numbere45684
JournalPLoS One
Volume7
Issue number9
DOIs
StatePublished - Sep 25 2012

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Triple Negative Breast Neoplasms
Epithelial-Mesenchymal Transition
phenotypic plasticity
breast neoplasms
Plasticity
Cells
Biological Sciences
Breast Neoplasms
Cell Line
Serum-Free Culture Media
Stem cells
Heterografts
cell lines
Tumors
Transcription Factors
Phenotype
Genes
cells
stem cells
Neoplastic Stem Cells

Cite this

Evidence for Phenotypic Plasticity in Aggressive Triple-Negative Breast Cancer : Human Biology Is Recapitulated by a Novel Model System. / Ostrander, Julie H; D'Amato, Nicholas C.; Bowie, Michelle L.; Sistrunk, Christopher; Borowsky, Alexander; Cardiff, Robert D.; Bell, Katie; Young, Lawrence J.T.; Simin, Karl; Bachelder, Robin E.; Delrow, Jeff; Dawson, Alyssa; Yee, Lisa D.; Mrózek, Krzysztof; Clay, Timothy M.; Osada, Takuya; Seewaldt, Victoria L.

In: PLoS One, Vol. 7, No. 9, e45684, 25.09.2012.

Research output: Contribution to journalArticle

Ostrander, JH, D'Amato, NC, Bowie, ML, Sistrunk, C, Borowsky, A, Cardiff, RD, Bell, K, Young, LJT, Simin, K, Bachelder, RE, Delrow, J, Dawson, A, Yee, LD, Mrózek, K, Clay, TM, Osada, T & Seewaldt, VL 2012, 'Evidence for Phenotypic Plasticity in Aggressive Triple-Negative Breast Cancer: Human Biology Is Recapitulated by a Novel Model System', PLoS One, vol. 7, no. 9, e45684. https://doi.org/10.1371/journal.pone.0045684
Ostrander, Julie H ; D'Amato, Nicholas C. ; Bowie, Michelle L. ; Sistrunk, Christopher ; Borowsky, Alexander ; Cardiff, Robert D. ; Bell, Katie ; Young, Lawrence J.T. ; Simin, Karl ; Bachelder, Robin E. ; Delrow, Jeff ; Dawson, Alyssa ; Yee, Lisa D. ; Mrózek, Krzysztof ; Clay, Timothy M. ; Osada, Takuya ; Seewaldt, Victoria L. / Evidence for Phenotypic Plasticity in Aggressive Triple-Negative Breast Cancer : Human Biology Is Recapitulated by a Novel Model System. In: PLoS One. 2012 ; Vol. 7, No. 9.
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