Evidence for multiple ribonucleases in crude extracts from cultured mosquito cells

Mary Ann Fritz, Ann Marie Fallon

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Crude extracts from Aedes albopictus (mosquito) cells appear to contain several ribonuclease activities, which can be differentiated on the basis of heat-stability, pH optima and the effects of divalent cations. Ribonuclease activities which can be detected on polyacrylamide gels differ with respect to molecular weight, and various subcellular fractions appear to have distinct ribonuclease activities. Zinc chloride and heparin are effective inhibitors of ribonuclease activity in crude extracts. These results provide useful criteria for further characterization of the major ribonucleases present in cultured mosquito cells.

Original languageEnglish (US)
Pages (from-to)817-825
Number of pages9
JournalInsect Biochemistry
Volume15
Issue number6
DOIs
StatePublished - 1985

Bibliographical note

Funding Information:
Acknowledgements--This work was supported by the UMDNJ Foundation and Grant AI-20385 from the National Institute of Allergy and Infectious Diseases. We

Keywords

  • Aedes albopictus
  • cultured mosquito cells
  • ribonuclease

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