Crude extracts from Aedes albopictus (mosquito) cells appear to contain several ribonuclease activities, which can be differentiated on the basis of heat-stability, pH optima and the effects of divalent cations. Ribonuclease activities which can be detected on polyacrylamide gels differ with respect to molecular weight, and various subcellular fractions appear to have distinct ribonuclease activities. Zinc chloride and heparin are effective inhibitors of ribonuclease activity in crude extracts. These results provide useful criteria for further characterization of the major ribonucleases present in cultured mosquito cells.
|Original language||English (US)|
|Number of pages||9|
|State||Published - 1985|
Bibliographical noteFunding Information:
Acknowledgements--This work was supported by the UMDNJ Foundation and Grant AI-20385 from the National Institute of Allergy and Infectious Diseases. We
- Aedes albopictus
- cultured mosquito cells