Evidence for dominant suppression of repeat-induced point mutation (RIP) in crosses with the wild-isolated Neurospora crassa strains Sugartown and Adiopodoume-7

Felicite K. Noubissi; K. Aparna; Kevin McCluskey; Durgadas P. Kasbekar

doi:10.1007/BF02728331

Evidence for dominant suppression of repeat-induced point mutation (RIP) in crosses with the wild-isolated Neurospora crassa strains Sugartown and Adiopodoume-7

Felicite K. Noubissi, K. Aparna, Kevin McCluskey, Durgadas P. Kasbekar

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A convenient assay to score repeat-induced point mutation (RIP) in Neurospora employs the erg-3 locus as a mutagenesis target. Using this assay we screened 132 wild-isolated Neurospora crassa strains for ability to dominantly suppress RIP. RIP was exceptionally inefficient in crosses with the wild isolates Sugartown (P0854) and Adiopodoume-7 (P4305), thereby suggesting the presence of dominant RIP suppressors in these strains. In other experiments, we found no evidence for dominant RIP suppression by the Spore killer haploty pes Sk-2 and Sk-3.

Original language	English (US)
Pages (from-to)	55-61
Number of pages	7
Journal	Journal of Genetics
Volume	80
Issue number	2
DOIs	https://doi.org/10.1007/BF02728331
State	Published - Aug 2001
Externally published	Yes

Bibliographical note

Funding Information:
generation of null mutants by-inredpuecaetd point mutation We thank Ashwin Bhat and Meenal Vyas for assisting ina ntdh ecomplementation by proteins chimeric for human lamin B ‘double-blind’ experiments. F. K. N. was supported byr eaceptor sequenMceicsr.o biolog1y45, 144–31451. fellowship from the Third World Organization of WomReanju i nN. B. 1994 Ascomycete Spore kilhlerorsm: oCsomal Science. elements that distort genetic ratios among the products of meiosisM. ycologia86, 461– 473. Raju N. B. 1996 Meiotic drive in fungi: chromosomal elements that cause fratricide and distort genetJi.c Graetnieo.st 7. 5, 287– 296. Bhat A. and Kasbekar D. P. 2001 Escape fro-mind ruecpeeda t Selker E. U. 1990e mPreiotic instability of repeated sequences point mutation of a g-seinzeed duplication Nine urospora in Neurospora cra.s Asannu. Rev. Gene.t 24, 597– 613. crassac rosses that are heterozygous for a larger chromSeonsgoumpeta S., Bhavani Prasanna T. and Kasbekar D. P. 1995 segment duplicatGioenn. etic1s57, 158–11590. Sterol 14,15 reductearsge-3 () mutants switch the phenotype Davis R. H. and De Serres J. F. 1970 Genemticic rao-nd of Neurospora crasfsraom sensitivity teo ttohmato saponin biological research techniquNeesu froosrp ora cras.s Ma ethods a-tomatine to sensitivity to the pea phytoalexin pisatin. Enzymo. lA17, 79– 143. Fungal Gen.eNt ewsle.t4t 2, 71– 72. Graia F., Lespinet O., Rimbault B., D-eCqhuaabrldat M., Singer M. J., Kuzminova E. A., Tharp A., Margolin B. S. and Coppin E. and Picard M. 2001 Genome quality control:S RelIkPer E. U. 1995 Different frequencies of RIP among early (repea-itnduced point mutation) comPeosd otosp or.a Mol. vs late ascospores Noefu rospora cras.s aFungal Gen.et Microbio.l 40, 586– 595. Newsle.t t42, 74– 75. Irelan J. T. ande lSker E. U. 1996 Gene silencing in -filamTeunrner B. C. 2001 Geographic distribution of Neurospora Spore tous fungi: RIP, MIP and quellJin. gG. ene.t 75, 313– killer strains and strains resistant to Fkuinllgi.ngG.e ne.t 324. Biol. 32, 93– 104. Kinsey J. A. 1989 Restricted distributionT aodf ttrhaen sposon Turner B. C., Perkins D. D. and Fairfield A. 2001 Neurospora in strains oNfe urospor. aCurr. Gene.t 15, 271– 275. fromnatural populations: A global Fstuundg.yG. ene.tBiol. Noubissi F. K., McCluskey K. and eKkaasrb D. P. 2000 Re-peat 32, 67– 92.

Keywords

Meiotic drive
Neurospora crassa
RIP
Spore killer

Publisher link

10.1007/BF02728331

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title = "Evidence for dominant suppression of repeat-induced point mutation (RIP) in crosses with the wild-isolated Neurospora crassa strains Sugartown and Adiopodoume-7",

abstract = "A convenient assay to score repeat-induced point mutation (RIP) in Neurospora employs the erg-3 locus as a mutagenesis target. Using this assay we screened 132 wild-isolated Neurospora crassa strains for ability to dominantly suppress RIP. RIP was exceptionally inefficient in crosses with the wild isolates Sugartown (P0854) and Adiopodoume-7 (P4305), thereby suggesting the presence of dominant RIP suppressors in these strains. In other experiments, we found no evidence for dominant RIP suppression by the Spore killer haploty pes Sk-2 and Sk-3.",

keywords = "Meiotic drive, Neurospora crassa, RIP, Spore killer",

author = "Noubissi, {Felicite K.} and K. Aparna and Kevin McCluskey and Kasbekar, {Durgadas P.}",

note = "Funding Information: generation of null mutants by-inredpuecaetd point mutation We thank Ashwin Bhat and Meenal Vyas for assisting ina ntdh ecomplementation by proteins chimeric for human lamin B {\textquoteleft}double-blind{\textquoteright} experiments. F. K. N. was supported byr eaceptor sequenMceicsr.o biolog1y45, 144–31451. fellowship from the Third World Organization of WomReanju i nN. B. 1994 Ascomycete Spore kilhlerorsm: oCsomal Science. elements that distort genetic ratios among the products of meiosisM. ycologia86, 461– 473. Raju N. B. 1996 Meiotic drive in fungi: chromosomal elements that cause fratricide and distort genetJi.c Graetnieo.st 7. 5, 287– 296. Bhat A. and Kasbekar D. P. 2001 Escape fro-mind ruecpeeda t Selker E. U. 1990e mPreiotic instability of repeated sequences point mutation of a g-seinzeed duplication Nine urospora in Neurospora cra.s Asannu. Rev. Gene.t 24, 597– 613. crassac rosses that are heterozygous for a larger chromSeonsgoumpeta S., Bhavani Prasanna T. and Kasbekar D. P. 1995 segment duplicatGioenn. etic1s57, 158–11590. Sterol 14,15 reductearsge-3 () mutants switch the phenotype Davis R. H. and De Serres J. F. 1970 Genemticic rao-nd of Neurospora crasfsraom sensitivity teo ttohmato saponin biological research techniquNeesu froosrp ora cras.s Ma ethods a-tomatine to sensitivity to the pea phytoalexin pisatin. Enzymo. lA17, 79– 143. Fungal Gen.eNt ewsle.t4t 2, 71– 72. Graia F., Lespinet O., Rimbault B., D-eCqhuaabrldat M., Singer M. J., Kuzminova E. A., Tharp A., Margolin B. S. and Coppin E. and Picard M. 2001 Genome quality control:S RelIkPer E. U. 1995 Different frequencies of RIP among early (repea-itnduced point mutation) comPeosd otosp or.a Mol. vs late ascospores Noefu rospora cras.s aFungal Gen.et Microbio.l 40, 586– 595. Newsle.t t42, 74– 75. Irelan J. T. ande lSker E. U. 1996 Gene silencing in -filamTeunrner B. C. 2001 Geographic distribution of Neurospora Spore tous fungi: RIP, MIP and quellJin. gG. ene.t 75, 313– killer strains and strains resistant to Fkuinllgi.ngG.e ne.t 324. Biol. 32, 93– 104. Kinsey J. A. 1989 Restricted distributionT aodf ttrhaen sposon Turner B. C., Perkins D. D. and Fairfield A. 2001 Neurospora in strains oNfe urospor. aCurr. Gene.t 15, 271– 275. fromnatural populations: A global Fstuundg.yG. ene.tBiol. Noubissi F. K., McCluskey K. and eKkaasrb D. P. 2000 Re-peat 32, 67– 92.",

year = "2001",

month = aug,

doi = "10.1007/BF02728331",

language = "English (US)",

volume = "80",

pages = "55--61",

journal = "Journal of Genetics",

issn = "0022-1333",

publisher = "Springer",

number = "2",

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T1 - Evidence for dominant suppression of repeat-induced point mutation (RIP) in crosses with the wild-isolated Neurospora crassa strains Sugartown and Adiopodoume-7

AU - Noubissi, Felicite K.

AU - Aparna, K.

AU - McCluskey, Kevin

AU - Kasbekar, Durgadas P.

N1 - Funding Information: generation of null mutants by-inredpuecaetd point mutation We thank Ashwin Bhat and Meenal Vyas for assisting ina ntdh ecomplementation by proteins chimeric for human lamin B ‘double-blind’ experiments. F. K. N. was supported byr eaceptor sequenMceicsr.o biolog1y45, 144–31451. fellowship from the Third World Organization of WomReanju i nN. B. 1994 Ascomycete Spore kilhlerorsm: oCsomal Science. elements that distort genetic ratios among the products of meiosisM. ycologia86, 461– 473. Raju N. B. 1996 Meiotic drive in fungi: chromosomal elements that cause fratricide and distort genetJi.c Graetnieo.st 7. 5, 287– 296. Bhat A. and Kasbekar D. P. 2001 Escape fro-mind ruecpeeda t Selker E. U. 1990e mPreiotic instability of repeated sequences point mutation of a g-seinzeed duplication Nine urospora in Neurospora cra.s Asannu. Rev. Gene.t 24, 597– 613. crassac rosses that are heterozygous for a larger chromSeonsgoumpeta S., Bhavani Prasanna T. and Kasbekar D. P. 1995 segment duplicatGioenn. etic1s57, 158–11590. Sterol 14,15 reductearsge-3 () mutants switch the phenotype Davis R. H. and De Serres J. F. 1970 Genemticic rao-nd of Neurospora crasfsraom sensitivity teo ttohmato saponin biological research techniquNeesu froosrp ora cras.s Ma ethods a-tomatine to sensitivity to the pea phytoalexin pisatin. Enzymo. lA17, 79– 143. Fungal Gen.eNt ewsle.t4t 2, 71– 72. Graia F., Lespinet O., Rimbault B., D-eCqhuaabrldat M., Singer M. J., Kuzminova E. A., Tharp A., Margolin B. S. and Coppin E. and Picard M. 2001 Genome quality control:S RelIkPer E. U. 1995 Different frequencies of RIP among early (repea-itnduced point mutation) comPeosd otosp or.a Mol. vs late ascospores Noefu rospora cras.s aFungal Gen.et Microbio.l 40, 586– 595. Newsle.t t42, 74– 75. Irelan J. T. ande lSker E. U. 1996 Gene silencing in -filamTeunrner B. C. 2001 Geographic distribution of Neurospora Spore tous fungi: RIP, MIP and quellJin. gG. ene.t 75, 313– killer strains and strains resistant to Fkuinllgi.ngG.e ne.t 324. Biol. 32, 93– 104. Kinsey J. A. 1989 Restricted distributionT aodf ttrhaen sposon Turner B. C., Perkins D. D. and Fairfield A. 2001 Neurospora in strains oNfe urospor. aCurr. Gene.t 15, 271– 275. fromnatural populations: A global Fstuundg.yG. ene.tBiol. Noubissi F. K., McCluskey K. and eKkaasrb D. P. 2000 Re-peat 32, 67– 92.

PY - 2001/8

Y1 - 2001/8

N2 - A convenient assay to score repeat-induced point mutation (RIP) in Neurospora employs the erg-3 locus as a mutagenesis target. Using this assay we screened 132 wild-isolated Neurospora crassa strains for ability to dominantly suppress RIP. RIP was exceptionally inefficient in crosses with the wild isolates Sugartown (P0854) and Adiopodoume-7 (P4305), thereby suggesting the presence of dominant RIP suppressors in these strains. In other experiments, we found no evidence for dominant RIP suppression by the Spore killer haploty pes Sk-2 and Sk-3.

AB - A convenient assay to score repeat-induced point mutation (RIP) in Neurospora employs the erg-3 locus as a mutagenesis target. Using this assay we screened 132 wild-isolated Neurospora crassa strains for ability to dominantly suppress RIP. RIP was exceptionally inefficient in crosses with the wild isolates Sugartown (P0854) and Adiopodoume-7 (P4305), thereby suggesting the presence of dominant RIP suppressors in these strains. In other experiments, we found no evidence for dominant RIP suppression by the Spore killer haploty pes Sk-2 and Sk-3.

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KW - RIP

KW - Spore killer

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Evidence for dominant suppression of repeat-induced point mutation (RIP) in crosses with the wild-isolated Neurospora crassa strains Sugartown and Adiopodoume-7

Abstract

Bibliographical note

Keywords

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