Abstract
Infiltration of Agrobacterium tumefaciens into intact plant leaves of N. benthamiana was used to test the efficiency of two virus-based silencing constructs conferring resistance to the closely related begomoviruses. The constructs contained the most conserved sequences of the coat protein (CP) gene and replication-associated protein (Rep) gene of Tomato yellow leaf curl Sardinia virus (Sicily strain) (TYLCSV-[Sic]). Both constructs formed a hairpin structure that enhanced the post-transcriptional gene-silencing mechanism. When agro-infiltrated plants were challenged separately with infectious viruses TYLCSV-[Sic] and Tomato yellow leaf curl virus (TYLCV), the plants showed resistance to TYLCSV-[Sic], but not to the related TYLCV.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 143-9 |
| Number of pages | 7 |
| Journal | Acta Virologica |
| Volume | 52 |
| Issue number | 3 |
| State | Published - 2008 |
Keywords
- Agriculture/methods
- Agrobacterium tumefaciens/genetics
- Begomovirus/genetics
- Capsid Proteins/genetics
- Solanum lycopersicum/virology
- Plant Diseases/virology
- Plant Leaves/virology
- Plants, Genetically Modified/genetics
- RNA Interference
- Tobacco/genetics
- Viral Proteins/genetics
PubMed: MeSH publication types
- Evaluation Study
- Journal Article
- Research Support, Non-U.S. Gov't