Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses

Mariana Kikuti; Laura B. Tauro; Patrícia S.S. Moreira; Leile Camila J. Nascimento; Moyra M. Portilho; Gúbio C. Soares; Scott C. Weaver; Mitermayer G. Reis; Uriel Kitron; Guilherme S. Ribeiro

doi:10.1016/j.ijid.2019.11.001

Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses

Mariana Kikuti, Laura B. Tauro, Patrícia S.S. Moreira, Leile Camila J. Nascimento, Moyra M. Portilho, Gúbio C. Soares, Scott C. Weaver, Mitermayer G. Reis, Uriel Kitron, Guilherme S. Ribeiro

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.

Original language	English (US)
Pages (from-to)	38-43
Number of pages	6
Journal	International Journal of Infectious Diseases
Volume	91
DOIs	https://doi.org/10.1016/j.ijid.2019.11.001
State	Published - Feb 2020

Bibliographical note

Funding Information:
This study was supported by the Brazilian National Council for Scientific and Technological Development (grants 400830/2013-2 and 440891/2016-7 to GSR; and scholarships to UK, MGR, and GSR); the Bahia Foundation for Research Support (grants PET0026/2013 , APP0044/2016 , and PET0022/2016 to GSR); the Coordination for the Improvement of Higher Education Personnel, Brazilian Ministry of Education (grant 88887.130746/2016-00 to GSR and scholarship to MK); the Oswaldo Cruz Foundation ; the Federal University of Bahia ; and the Department of Science and Technology, Secretariat of Science, Technology and Strategic Inputs, Brazilian Ministry of Health . The funding sources had no role in study design; in the collection, analysis and interpretation of data; in the writing of the paper; and in the decision to submit the article for publication.

Publisher Copyright:
© 2019 The Author(s)

Keywords

Accuracy
Chikungunya virus
Diagnostic performance
ELISA
Enzyme-linked immunosorbent assay
Sensitivity and specificity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijid.2019.11.001

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Kikuti, M., Tauro, L. B., Moreira, P. S. S., Nascimento, L. C. J., Portilho, M. M., Soares, G. C., Weaver, S. C., Reis, M. G., Kitron, U., & Ribeiro, G. S. (2020). Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. International Journal of Infectious Diseases, 91, 38-43. https://doi.org/10.1016/j.ijid.2019.11.001

Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. / Kikuti, Mariana; Tauro, Laura B.; Moreira, Patrícia S.S. et al.
In: International Journal of Infectious Diseases, Vol. 91, 02.2020, p. 38-43.

Research output: Contribution to journal › Article › peer-review

Kikuti, M, Tauro, LB, Moreira, PSS, Nascimento, LCJ, Portilho, MM, Soares, GC, Weaver, SC, Reis, MG, Kitron, U & Ribeiro, GS 2020, 'Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses', International Journal of Infectious Diseases, vol. 91, pp. 38-43. https://doi.org/10.1016/j.ijid.2019.11.001

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title = "Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses",

abstract = "Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests{\textquoteright} accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests{\textquoteright} sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.",

keywords = "Accuracy, Chikungunya virus, Diagnostic performance, ELISA, Enzyme-linked immunosorbent assay, Sensitivity and specificity",

author = "Mariana Kikuti and Tauro, {Laura B.} and Moreira, {Patr{\'i}cia S.S.} and Nascimento, {Leile Camila J.} and Portilho, {Moyra M.} and Soares, {G{\'u}bio C.} and Weaver, {Scott C.} and Reis, {Mitermayer G.} and Uriel Kitron and Ribeiro, {Guilherme S.}",

note = "Funding Information: This study was supported by the Brazilian National Council for Scientific and Technological Development (grants 400830/2013-2 and 440891/2016-7 to GSR; and scholarships to UK, MGR, and GSR); the Bahia Foundation for Research Support (grants PET0026/2013 , APP0044/2016 , and PET0022/2016 to GSR); the Coordination for the Improvement of Higher Education Personnel, Brazilian Ministry of Education (grant 88887.130746/2016-00 to GSR and scholarship to MK); the Oswaldo Cruz Foundation ; the Federal University of Bahia ; and the Department of Science and Technology, Secretariat of Science, Technology and Strategic Inputs, Brazilian Ministry of Health . The funding sources had no role in study design; in the collection, analysis and interpretation of data; in the writing of the paper; and in the decision to submit the article for publication. Publisher Copyright: {\textcopyright} 2019 The Author(s)",

year = "2020",

month = feb,

doi = "10.1016/j.ijid.2019.11.001",

language = "English (US)",

volume = "91",

pages = "38--43",

journal = "International Journal of Infectious Diseases",

issn = "1201-9712",

publisher = "Elsevier",

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TY - JOUR

T1 - Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses

AU - Kikuti, Mariana

AU - Tauro, Laura B.

AU - Moreira, Patrícia S.S.

AU - Nascimento, Leile Camila J.

AU - Portilho, Moyra M.

AU - Soares, Gúbio C.

AU - Weaver, Scott C.

AU - Reis, Mitermayer G.

AU - Kitron, Uriel

AU - Ribeiro, Guilherme S.

N1 - Funding Information: This study was supported by the Brazilian National Council for Scientific and Technological Development (grants 400830/2013-2 and 440891/2016-7 to GSR; and scholarships to UK, MGR, and GSR); the Bahia Foundation for Research Support (grants PET0026/2013 , APP0044/2016 , and PET0022/2016 to GSR); the Coordination for the Improvement of Higher Education Personnel, Brazilian Ministry of Education (grant 88887.130746/2016-00 to GSR and scholarship to MK); the Oswaldo Cruz Foundation ; the Federal University of Bahia ; and the Department of Science and Technology, Secretariat of Science, Technology and Strategic Inputs, Brazilian Ministry of Health . The funding sources had no role in study design; in the collection, analysis and interpretation of data; in the writing of the paper; and in the decision to submit the article for publication. Publisher Copyright: © 2019 The Author(s)

PY - 2020/2

Y1 - 2020/2

N2 - Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.

AB - Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.

KW - Accuracy

KW - Chikungunya virus

KW - Diagnostic performance

KW - ELISA

KW - Enzyme-linked immunosorbent assay

KW - Sensitivity and specificity

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Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses

Abstract

Bibliographical note

Keywords

UN SDGs

Publisher link

Find It

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