Evaluation of the thermo-assisted drying and decontamination system for sanitation of a full-size transport vehicle contaminated with porcine reproductive and respiratory syndrome virus

S. A. Dee, M. Torremorell, R. Thompson, J. P. Cano, J. Deen, C. Pijoan

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Objectives: To determine whether full-size livestock trailers serve as a source of porcine reproductive and respiratory syndrome virus (PRRSV) infection for naive swine, to test the thermo-assisted drying and decontamination (TADD) system in full-size trailers, and to test a method to quantify moisture in trailers post TADD. Methods: Five donor pigs infected with PRRSV (Day 0) were confined within a full-size trailer for a 4-hour contamination period on Days 3 to 7 (five replicates). After each contamination period, a group of five PRRSV-naive recipients (25 pigs total) replaced the donor pigs in the pen for 4 hours. The pen was not washed or sanitized between donor and recipient groups. Recipients were tested for PRRSV by reverse transcriptase-polymerase chain reaction (RT-PCR) post exposure. To test the efficacy of TADD, the trailer was washed, 15 pre-selected interior sites were contaminated with PRRSV, and the TADD system was applied for 2 hours. Swabs collected from the sites at 0, 60, and 120 minutes post treatment were tested by RT-PCR and swine bioassay. A drag-swab technique was developed to measure residual moisture post TADD. Results: Infection of recipient pigs was observed in three replicates. At 0 and 60 but not 120 minutes post TADD, trailer swabs were RT-PCR-positive. Moisture in the drag swabs differed (P < .001) at 0, 60, and 120 minutes post TADD. Implications: Under the conditions of this study, PRRSV-contaminated transport vehicles are a source of infection for naive pigs. The TADD system is efficacious for decontaminating and drying PRRSV-positive trailers.

Original languageEnglish (US)
Pages (from-to)12-18
Number of pages7
JournalJournal of Swine Health and Production
Volume15
Issue number1
StatePublished - Jan 1 2007

Keywords

  • Biosecurity
  • Porcine reproductive and respiratory syndrome virus
  • Swine
  • Thermo-assisted drying and decontamination system

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