Evaluation of the performance of cellular and serological diagnostic tests for the diagnosis of tuberculosis in an alpaca (Vicugna pacos) herd naturally infected with Mycobacterium bovis

Javier Bezos, Carmen Casal, Julio Álvarez, Alberto Díez-Guerrier, Antonio Rodríguez-Bertos, Beatriz Romero, Paloma Rueda, Lissette López, Lucas Domínguez, Lucía De Juan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tuberculosis (TB) in llamas and alpacas has gained importance in recent years since they are imported into the European Union mainly for serving as pets and for production of natural fibre. The intradermal tuberculin test has been widely used for diagnosis of TB in these species showing lack of sensitivity (Se) although little information has been previously reported evaluating the effect on its performance of different PPD inoculation sites and time of readings. Moreover, different cost-effective serological assays have been developed in the recent years for TB diagnosis in camelids obtaining a variety of results and, for this reason, new assays still being developed. The main objectives of this study were: (1) to evaluate the performance of the intradermal tuberculin test using different inoculation sites (axillary, prescapular and cervical) and times of reading (72 and 120. h) and (2) to test a novel serological assay based on MPB83 antigen in a Mycobacterium bovis naturally infected alpaca herd in Spain. In regards to skin test, single intradermal tuberculin (SIT) test at the prescapular site and reading at 72. h showed the highest proportion of test-positive-culture positive animals among all culture positive animals (T+/C+), ranging from 53.8% (95% CI, 37.2-69.9) to 80% (95% CI, 44.4-97.5) using a more stringent interpretation than typically prescribed although, in general, low T+/C+ was achieved using both SIT and single comparative intradermal tuberculin (SCIT) tests alone. T+/C+ of the serological assay increased using samples collected 15-30 days after PPD injection [76.9% (95% CI, 60.7-88.9) - 100% (95% CI, 69.2-100)]. The best results of T+/C+ were obtained applying in parallel the most sensitive SIT test and serology using samples collected 15-30 days after PPD inoculation [90% (95% CI, 55.5-99.7)-100% (95% CI, 69.2-100)]. Therefore implementation of serology in parallel with the most sensitive skin test could maximize the detection of infected animals.

Original languageEnglish (US)
Pages (from-to)304-313
Number of pages10
JournalPreventive Veterinary Medicine
Volume111
Issue number3-4
DOIs
StatePublished - Sep 1 2013

Fingerprint

New World Camelids
Intradermal Tests
Tuberculin Test
alpacas
Mycobacterium bovis
Serologic Tests
tuberculin
Routine Diagnostic Tests
tuberculosis
diagnostic techniques
Tuberculin
Tuberculosis
herds
Reading
Serology
Skin Tests
testing
skin tests
assays
Pets

Keywords

  • Alpaca
  • Diagnosis
  • Intradermal test
  • Serology
  • Tuberculosis

Cite this

Evaluation of the performance of cellular and serological diagnostic tests for the diagnosis of tuberculosis in an alpaca (Vicugna pacos) herd naturally infected with Mycobacterium bovis. / Bezos, Javier; Casal, Carmen; Álvarez, Julio; Díez-Guerrier, Alberto; Rodríguez-Bertos, Antonio; Romero, Beatriz; Rueda, Paloma; López, Lissette; Domínguez, Lucas; De Juan, Lucía.

In: Preventive Veterinary Medicine, Vol. 111, No. 3-4, 01.09.2013, p. 304-313.

Research output: Contribution to journalArticle

Bezos, J, Casal, C, Álvarez, J, Díez-Guerrier, A, Rodríguez-Bertos, A, Romero, B, Rueda, P, López, L, Domínguez, L & De Juan, L 2013, 'Evaluation of the performance of cellular and serological diagnostic tests for the diagnosis of tuberculosis in an alpaca (Vicugna pacos) herd naturally infected with Mycobacterium bovis', Preventive Veterinary Medicine, vol. 111, no. 3-4, pp. 304-313. https://doi.org/10.1016/j.prevetmed.2013.05.013
Bezos, Javier ; Casal, Carmen ; Álvarez, Julio ; Díez-Guerrier, Alberto ; Rodríguez-Bertos, Antonio ; Romero, Beatriz ; Rueda, Paloma ; López, Lissette ; Domínguez, Lucas ; De Juan, Lucía. / Evaluation of the performance of cellular and serological diagnostic tests for the diagnosis of tuberculosis in an alpaca (Vicugna pacos) herd naturally infected with Mycobacterium bovis. In: Preventive Veterinary Medicine. 2013 ; Vol. 111, No. 3-4. pp. 304-313.
@article{167e72dd9e4e423d9505d9f698f6f8de,
title = "Evaluation of the performance of cellular and serological diagnostic tests for the diagnosis of tuberculosis in an alpaca (Vicugna pacos) herd naturally infected with Mycobacterium bovis",
abstract = "Tuberculosis (TB) in llamas and alpacas has gained importance in recent years since they are imported into the European Union mainly for serving as pets and for production of natural fibre. The intradermal tuberculin test has been widely used for diagnosis of TB in these species showing lack of sensitivity (Se) although little information has been previously reported evaluating the effect on its performance of different PPD inoculation sites and time of readings. Moreover, different cost-effective serological assays have been developed in the recent years for TB diagnosis in camelids obtaining a variety of results and, for this reason, new assays still being developed. The main objectives of this study were: (1) to evaluate the performance of the intradermal tuberculin test using different inoculation sites (axillary, prescapular and cervical) and times of reading (72 and 120. h) and (2) to test a novel serological assay based on MPB83 antigen in a Mycobacterium bovis naturally infected alpaca herd in Spain. In regards to skin test, single intradermal tuberculin (SIT) test at the prescapular site and reading at 72. h showed the highest proportion of test-positive-culture positive animals among all culture positive animals (T+/C+), ranging from 53.8{\%} (95{\%} CI, 37.2-69.9) to 80{\%} (95{\%} CI, 44.4-97.5) using a more stringent interpretation than typically prescribed although, in general, low T+/C+ was achieved using both SIT and single comparative intradermal tuberculin (SCIT) tests alone. T+/C+ of the serological assay increased using samples collected 15-30 days after PPD injection [76.9{\%} (95{\%} CI, 60.7-88.9) - 100{\%} (95{\%} CI, 69.2-100)]. The best results of T+/C+ were obtained applying in parallel the most sensitive SIT test and serology using samples collected 15-30 days after PPD inoculation [90{\%} (95{\%} CI, 55.5-99.7)-100{\%} (95{\%} CI, 69.2-100)]. Therefore implementation of serology in parallel with the most sensitive skin test could maximize the detection of infected animals.",
keywords = "Alpaca, Diagnosis, Intradermal test, Serology, Tuberculosis",
author = "Javier Bezos and Carmen Casal and Julio {\'A}lvarez and Alberto D{\'i}ez-Guerrier and Antonio Rodr{\'i}guez-Bertos and Beatriz Romero and Paloma Rueda and Lissette L{\'o}pez and Lucas Dom{\'i}nguez and {De Juan}, Luc{\'i}a",
year = "2013",
month = "9",
day = "1",
doi = "10.1016/j.prevetmed.2013.05.013",
language = "English (US)",
volume = "111",
pages = "304--313",
journal = "Preventive Veterinary Medicine",
issn = "0167-5877",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Evaluation of the performance of cellular and serological diagnostic tests for the diagnosis of tuberculosis in an alpaca (Vicugna pacos) herd naturally infected with Mycobacterium bovis

AU - Bezos, Javier

AU - Casal, Carmen

AU - Álvarez, Julio

AU - Díez-Guerrier, Alberto

AU - Rodríguez-Bertos, Antonio

AU - Romero, Beatriz

AU - Rueda, Paloma

AU - López, Lissette

AU - Domínguez, Lucas

AU - De Juan, Lucía

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Tuberculosis (TB) in llamas and alpacas has gained importance in recent years since they are imported into the European Union mainly for serving as pets and for production of natural fibre. The intradermal tuberculin test has been widely used for diagnosis of TB in these species showing lack of sensitivity (Se) although little information has been previously reported evaluating the effect on its performance of different PPD inoculation sites and time of readings. Moreover, different cost-effective serological assays have been developed in the recent years for TB diagnosis in camelids obtaining a variety of results and, for this reason, new assays still being developed. The main objectives of this study were: (1) to evaluate the performance of the intradermal tuberculin test using different inoculation sites (axillary, prescapular and cervical) and times of reading (72 and 120. h) and (2) to test a novel serological assay based on MPB83 antigen in a Mycobacterium bovis naturally infected alpaca herd in Spain. In regards to skin test, single intradermal tuberculin (SIT) test at the prescapular site and reading at 72. h showed the highest proportion of test-positive-culture positive animals among all culture positive animals (T+/C+), ranging from 53.8% (95% CI, 37.2-69.9) to 80% (95% CI, 44.4-97.5) using a more stringent interpretation than typically prescribed although, in general, low T+/C+ was achieved using both SIT and single comparative intradermal tuberculin (SCIT) tests alone. T+/C+ of the serological assay increased using samples collected 15-30 days after PPD injection [76.9% (95% CI, 60.7-88.9) - 100% (95% CI, 69.2-100)]. The best results of T+/C+ were obtained applying in parallel the most sensitive SIT test and serology using samples collected 15-30 days after PPD inoculation [90% (95% CI, 55.5-99.7)-100% (95% CI, 69.2-100)]. Therefore implementation of serology in parallel with the most sensitive skin test could maximize the detection of infected animals.

AB - Tuberculosis (TB) in llamas and alpacas has gained importance in recent years since they are imported into the European Union mainly for serving as pets and for production of natural fibre. The intradermal tuberculin test has been widely used for diagnosis of TB in these species showing lack of sensitivity (Se) although little information has been previously reported evaluating the effect on its performance of different PPD inoculation sites and time of readings. Moreover, different cost-effective serological assays have been developed in the recent years for TB diagnosis in camelids obtaining a variety of results and, for this reason, new assays still being developed. The main objectives of this study were: (1) to evaluate the performance of the intradermal tuberculin test using different inoculation sites (axillary, prescapular and cervical) and times of reading (72 and 120. h) and (2) to test a novel serological assay based on MPB83 antigen in a Mycobacterium bovis naturally infected alpaca herd in Spain. In regards to skin test, single intradermal tuberculin (SIT) test at the prescapular site and reading at 72. h showed the highest proportion of test-positive-culture positive animals among all culture positive animals (T+/C+), ranging from 53.8% (95% CI, 37.2-69.9) to 80% (95% CI, 44.4-97.5) using a more stringent interpretation than typically prescribed although, in general, low T+/C+ was achieved using both SIT and single comparative intradermal tuberculin (SCIT) tests alone. T+/C+ of the serological assay increased using samples collected 15-30 days after PPD injection [76.9% (95% CI, 60.7-88.9) - 100% (95% CI, 69.2-100)]. The best results of T+/C+ were obtained applying in parallel the most sensitive SIT test and serology using samples collected 15-30 days after PPD inoculation [90% (95% CI, 55.5-99.7)-100% (95% CI, 69.2-100)]. Therefore implementation of serology in parallel with the most sensitive skin test could maximize the detection of infected animals.

KW - Alpaca

KW - Diagnosis

KW - Intradermal test

KW - Serology

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=84881311802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881311802&partnerID=8YFLogxK

U2 - 10.1016/j.prevetmed.2013.05.013

DO - 10.1016/j.prevetmed.2013.05.013

M3 - Article

VL - 111

SP - 304

EP - 313

JO - Preventive Veterinary Medicine

JF - Preventive Veterinary Medicine

SN - 0167-5877

IS - 3-4

ER -