Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations

Javier Bezos, Julio Álvarez, Olga Mínguez, Sergio Marqués, Olegario Martín, Virginia Vigo, Carmen Pieltain, Beatriz Romero, Sabrina Rodríguez, Carmen Casal, Ana Mateos, Lucas Domínguez, Lucía De Juan

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


The aim of this study was to evaluate the specificity of the most widely used tuberculosis (TB) diagnostic tests, single intradermal tuberculin (SIT) and single comparative intradermal tuberculin (SCIT) tests and interferon-gamma (IFN-γ) assay in 937 animals from eight TB-free caprine flocks under different epidemiological situations. Maximum specificity was found using SCIT test (99.4-100% depending on the interpretation criteria) while SIT test and IFN-γ assay showed a slightly lower overall specificity (97.6-99.2% and 96.4-98.4% respectively). Specificity of the SIT test in a Corynebacterium pseudotuberculosis infected flock was significantly (. P<0.05) lower if a severe interpretation criterion was applied. Similarly, specificity values of SIT test and particularly IFN-γ assay in a paratuberculosis (PTB)-vaccinated flock were lower than those observed in non-vaccinated flocks. Higher proportion of false positive reactors to TB tests (SIT and IFN-γ assay) were observed among animals positive in the PTB-ELISA in PTB vaccinated flock. These results demonstrate that TB diagnostic tests show an adequate specificity when performed in goats from TB-free flocks in most situations. However, certain factors such as C. pseudotuberculosis infection and paratuberculosis vaccination can have a negative impact in the most sensitive tests.

Original languageEnglish (US)
Pages (from-to)636-640
Number of pages5
JournalResearch in veterinary science
Issue number2
StatePublished - Oct 2012

Bibliographical note

Funding Information:
This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology and the Ministry of Environment and Rural and Marine Affairs and the European Project FP7-KBBE-2007-1 “Strategies for the eradication of bovine tuberculosis (TB-STEP)”. We wish to thank The National Animal Health Authorities, in particular J.L. Sáez-Llorente, for their continuous encouragement. We are grateful to Sergio González for technical assistance.


  • Diagnosis
  • Goat
  • Paratuberculosis vaccination
  • Pseudotuberculosis
  • Test specificity
  • Tuberculosis


Dive into the research topics of 'Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations'. Together they form a unique fingerprint.

Cite this