Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations

Javier Bezos; Julio Álvarez; Olga Mínguez; Sergio Marqués; Olegario Martín; Virginia Vigo; Carmen Pieltain; Beatriz Romero; Sabrina Rodríguez; Carmen Casal; Ana Mateos; Lucas Domínguez; Lucía De Juan

doi:10.1016/j.rvsc.2011.10.009

Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations

Javier Bezos, Julio Álvarez, Olga Mínguez, Sergio Marqués, Olegario Martín, Virginia Vigo, Carmen Pieltain, Beatriz Romero, Sabrina Rodríguez, Carmen Casal, Ana Mateos, Lucas Domínguez, Lucía De Juan

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The aim of this study was to evaluate the specificity of the most widely used tuberculosis (TB) diagnostic tests, single intradermal tuberculin (SIT) and single comparative intradermal tuberculin (SCIT) tests and interferon-gamma (IFN-γ) assay in 937 animals from eight TB-free caprine flocks under different epidemiological situations. Maximum specificity was found using SCIT test (99.4-100% depending on the interpretation criteria) while SIT test and IFN-γ assay showed a slightly lower overall specificity (97.6-99.2% and 96.4-98.4% respectively). Specificity of the SIT test in a Corynebacterium pseudotuberculosis infected flock was significantly (. P<0.05) lower if a severe interpretation criterion was applied. Similarly, specificity values of SIT test and particularly IFN-γ assay in a paratuberculosis (PTB)-vaccinated flock were lower than those observed in non-vaccinated flocks. Higher proportion of false positive reactors to TB tests (SIT and IFN-γ assay) were observed among animals positive in the PTB-ELISA in PTB vaccinated flock. These results demonstrate that TB diagnostic tests show an adequate specificity when performed in goats from TB-free flocks in most situations. However, certain factors such as C. pseudotuberculosis infection and paratuberculosis vaccination can have a negative impact in the most sensitive tests.

Original language	English (US)
Pages (from-to)	636-640
Number of pages	5
Journal	Research in veterinary science
Volume	93
Issue number	2
DOIs	https://doi.org/10.1016/j.rvsc.2011.10.009
State	Published - Oct 2012
Externally published	Yes

Bibliographical note

Funding Information:
This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology and the Ministry of Environment and Rural and Marine Affairs and the European Project FP7-KBBE-2007-1 “Strategies for the eradication of bovine tuberculosis (TB-STEP)”. We wish to thank The National Animal Health Authorities, in particular J.L. Sáez-Llorente, for their continuous encouragement. We are grateful to Sergio González for technical assistance.

Keywords

Diagnosis
Goat
Paratuberculosis vaccination
Pseudotuberculosis
Test specificity
Tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.rvsc.2011.10.009

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Bezos, J., Álvarez, J., Mínguez, O., Marqués, S., Martín, O., Vigo, V., Pieltain, C., Romero, B., Rodríguez, S., Casal, C., Mateos, A., Domínguez, L., & De Juan, L. (2012). Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations. Research in veterinary science, 93(2), 636-640. https://doi.org/10.1016/j.rvsc.2011.10.009

Bezos, J, Álvarez, J, Mínguez, O, Marqués, S, Martín, O, Vigo, V, Pieltain, C, Romero, B, Rodríguez, S, Casal, C, Mateos, A, Domínguez, L & De Juan, L 2012, 'Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations', Research in veterinary science, vol. 93, no. 2, pp. 636-640. https://doi.org/10.1016/j.rvsc.2011.10.009

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abstract = "The aim of this study was to evaluate the specificity of the most widely used tuberculosis (TB) diagnostic tests, single intradermal tuberculin (SIT) and single comparative intradermal tuberculin (SCIT) tests and interferon-gamma (IFN-γ) assay in 937 animals from eight TB-free caprine flocks under different epidemiological situations. Maximum specificity was found using SCIT test (99.4-100% depending on the interpretation criteria) while SIT test and IFN-γ assay showed a slightly lower overall specificity (97.6-99.2% and 96.4-98.4% respectively). Specificity of the SIT test in a Corynebacterium pseudotuberculosis infected flock was significantly (. P<0.05) lower if a severe interpretation criterion was applied. Similarly, specificity values of SIT test and particularly IFN-γ assay in a paratuberculosis (PTB)-vaccinated flock were lower than those observed in non-vaccinated flocks. Higher proportion of false positive reactors to TB tests (SIT and IFN-γ assay) were observed among animals positive in the PTB-ELISA in PTB vaccinated flock. These results demonstrate that TB diagnostic tests show an adequate specificity when performed in goats from TB-free flocks in most situations. However, certain factors such as C. pseudotuberculosis infection and paratuberculosis vaccination can have a negative impact in the most sensitive tests.",

keywords = "Diagnosis, Goat, Paratuberculosis vaccination, Pseudotuberculosis, Test specificity, Tuberculosis",

author = "Javier Bezos and Julio {\'A}lvarez and Olga M{\'i}nguez and Sergio Marqu{\'e}s and Olegario Mart{\'i}n and Virginia Vigo and Carmen Pieltain and Beatriz Romero and Sabrina Rodr{\'i}guez and Carmen Casal and Ana Mateos and Lucas Dom{\'i}nguez and {De Juan}, Luc{\'i}a",

note = "Funding Information: This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology and the Ministry of Environment and Rural and Marine Affairs and the European Project FP7-KBBE-2007-1 “Strategies for the eradication of bovine tuberculosis (TB-STEP)”. We wish to thank The National Animal Health Authorities, in particular J.L. S{\'a}ez-Llorente, for their continuous encouragement. We are grateful to Sergio Gonz{\'a}lez for technical assistance.",

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AU - Bezos, Javier

AU - Álvarez, Julio

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AU - Marqués, Sergio

AU - Martín, Olegario

AU - Vigo, Virginia

AU - Pieltain, Carmen

AU - Romero, Beatriz

AU - Rodríguez, Sabrina

AU - Casal, Carmen

AU - Mateos, Ana

AU - Domínguez, Lucas

AU - De Juan, Lucía

N1 - Funding Information: This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology and the Ministry of Environment and Rural and Marine Affairs and the European Project FP7-KBBE-2007-1 “Strategies for the eradication of bovine tuberculosis (TB-STEP)”. We wish to thank The National Animal Health Authorities, in particular J.L. Sáez-Llorente, for their continuous encouragement. We are grateful to Sergio González for technical assistance.

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N2 - The aim of this study was to evaluate the specificity of the most widely used tuberculosis (TB) diagnostic tests, single intradermal tuberculin (SIT) and single comparative intradermal tuberculin (SCIT) tests and interferon-gamma (IFN-γ) assay in 937 animals from eight TB-free caprine flocks under different epidemiological situations. Maximum specificity was found using SCIT test (99.4-100% depending on the interpretation criteria) while SIT test and IFN-γ assay showed a slightly lower overall specificity (97.6-99.2% and 96.4-98.4% respectively). Specificity of the SIT test in a Corynebacterium pseudotuberculosis infected flock was significantly (. P<0.05) lower if a severe interpretation criterion was applied. Similarly, specificity values of SIT test and particularly IFN-γ assay in a paratuberculosis (PTB)-vaccinated flock were lower than those observed in non-vaccinated flocks. Higher proportion of false positive reactors to TB tests (SIT and IFN-γ assay) were observed among animals positive in the PTB-ELISA in PTB vaccinated flock. These results demonstrate that TB diagnostic tests show an adequate specificity when performed in goats from TB-free flocks in most situations. However, certain factors such as C. pseudotuberculosis infection and paratuberculosis vaccination can have a negative impact in the most sensitive tests.

AB - The aim of this study was to evaluate the specificity of the most widely used tuberculosis (TB) diagnostic tests, single intradermal tuberculin (SIT) and single comparative intradermal tuberculin (SCIT) tests and interferon-gamma (IFN-γ) assay in 937 animals from eight TB-free caprine flocks under different epidemiological situations. Maximum specificity was found using SCIT test (99.4-100% depending on the interpretation criteria) while SIT test and IFN-γ assay showed a slightly lower overall specificity (97.6-99.2% and 96.4-98.4% respectively). Specificity of the SIT test in a Corynebacterium pseudotuberculosis infected flock was significantly (. P<0.05) lower if a severe interpretation criterion was applied. Similarly, specificity values of SIT test and particularly IFN-γ assay in a paratuberculosis (PTB)-vaccinated flock were lower than those observed in non-vaccinated flocks. Higher proportion of false positive reactors to TB tests (SIT and IFN-γ assay) were observed among animals positive in the PTB-ELISA in PTB vaccinated flock. These results demonstrate that TB diagnostic tests show an adequate specificity when performed in goats from TB-free flocks in most situations. However, certain factors such as C. pseudotuberculosis infection and paratuberculosis vaccination can have a negative impact in the most sensitive tests.

KW - Diagnosis

KW - Goat

KW - Paratuberculosis vaccination

KW - Pseudotuberculosis

KW - Test specificity

KW - Tuberculosis

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Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations

Abstract

Bibliographical note

Keywords

UN SDGs

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