Evaluation of Methods for the Preparation of HL‐A Antigen‐Poor Blood

H. F. Polesky, J. McCullough, M. A. Helgeson, C. Nelson

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Abstract

More than 85 per cent of the leukocytes are removed from blood by dextran or Plasmagel sedimentation and by both high and low glycerol freeze‐thaw technics. Larger numbers of white cells and platelets remain in bloods processed by inverted centrifugation, saline washing, and agglomeration without freezing. Cytoglomeration is the least efficient method of removing HL‐A antigens from frozen blood. Units prepared by this and other freezing methods contain nonviable leukocytes which may or may not be antigenic. 1973 AABB

Original languageEnglish (US)
Pages (from-to)383-387
Number of pages5
JournalTransfusion
Volume13
Issue number6
DOIs
StatePublished - 1973

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    Polesky, H. F., McCullough, J., Helgeson, M. A., & Nelson, C. (1973). Evaluation of Methods for the Preparation of HL‐A Antigen‐Poor Blood. Transfusion, 13(6), 383-387. https://doi.org/10.1111/j.1537-2995.1973.tb04464.x