Evaluation of Methods for the Preparation of HL‐A Antigen‐Poor Blood

H. F. Polesky; J. McCullough; M. A. Helgeson; C. Nelson

doi:10.1111/j.1537-2995.1973.tb04464.x

Evaluation of Methods for the Preparation of HL‐A Antigen‐Poor Blood

H. F. Polesky, J. McCullough, M. A. Helgeson, C. Nelson

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

More than 85 per cent of the leukocytes are removed from blood by dextran or Plasmagel sedimentation and by both high and low glycerol freeze‐thaw technics. Larger numbers of white cells and platelets remain in bloods processed by inverted centrifugation, saline washing, and agglomeration without freezing. Cytoglomeration is the least efficient method of removing HL‐A antigens from frozen blood. Units prepared by this and other freezing methods contain nonviable leukocytes which may or may not be antigenic. 1973 AABB

Original language	English (US)
Pages (from-to)	383-387
Number of pages	5
Journal	Transfusion
Volume	13
Issue number	6
DOIs	https://doi.org/10.1111/j.1537-2995.1973.tb04464.x
State	Published - 1973

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title = "Evaluation of Methods for the Preparation of HL‐A Antigen‐Poor Blood",

abstract = "More than 85 per cent of the leukocytes are removed from blood by dextran or Plasmagel sedimentation and by both high and low glycerol freeze‐thaw technics. Larger numbers of white cells and platelets remain in bloods processed by inverted centrifugation, saline washing, and agglomeration without freezing. Cytoglomeration is the least efficient method of removing HL‐A antigens from frozen blood. Units prepared by this and other freezing methods contain nonviable leukocytes which may or may not be antigenic. 1973 AABB",

author = "Polesky, {H. F.} and J. McCullough and Helgeson, {M. A.} and C. Nelson",

year = "1973",

doi = "10.1111/j.1537-2995.1973.tb04464.x",

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pages = "383--387",

journal = "Transfusion",

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AU - Polesky, H. F.

AU - McCullough, J.

AU - Helgeson, M. A.

AU - Nelson, C.

PY - 1973

Y1 - 1973

N2 - More than 85 per cent of the leukocytes are removed from blood by dextran or Plasmagel sedimentation and by both high and low glycerol freeze‐thaw technics. Larger numbers of white cells and platelets remain in bloods processed by inverted centrifugation, saline washing, and agglomeration without freezing. Cytoglomeration is the least efficient method of removing HL‐A antigens from frozen blood. Units prepared by this and other freezing methods contain nonviable leukocytes which may or may not be antigenic. 1973 AABB

AB - More than 85 per cent of the leukocytes are removed from blood by dextran or Plasmagel sedimentation and by both high and low glycerol freeze‐thaw technics. Larger numbers of white cells and platelets remain in bloods processed by inverted centrifugation, saline washing, and agglomeration without freezing. Cytoglomeration is the least efficient method of removing HL‐A antigens from frozen blood. Units prepared by this and other freezing methods contain nonviable leukocytes which may or may not be antigenic. 1973 AABB

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JO - Transfusion

JF - Transfusion

SN - 0041-1132

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ER -

Evaluation of Methods for the Preparation of HL‐A Antigen‐Poor Blood

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