Evaluation of intracellular lipid bodies in Chlamydomonas reinhardtii strains by flow cytometry

Natarajan Velmurugan, Minji Sung, Sung Sun Yim, Min S. Park, Ji Won Yang, Ki Jun Jeong

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43 Scopus citations

Abstract

A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIPY 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to grow after single cell sorting using FACS, Nile Red stained cells failed to recover from sorting. In addition, a comprehensive study was performed to establish a quantitative baseline for future studies for either lipid accumulation and/or microalgal growth by measuring various parameters such as cell count, size, fatty acid contents/composition, and optical/confocal images of the wild type and mutant.

Original languageEnglish (US)
Pages (from-to)30-37
Number of pages8
JournalBioresource Technology
Volume138
DOIs
StatePublished - Jun 2013

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science, and Technology ( ABC-2010-0029728 and ABC-2012-053891 ). N. Velmurugan was supported by the BK21 Post-Doctoral Research Fund and Min S. Park was supported by the Brain Pool Program funded by the Korean Federation of Science and Technology Societies Grant by Korea Government (MEST, Basic Research Promotion Fund).

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • BODIPY 505/515
  • Chlamydomonas reinhardtii
  • Fluorescence-activated cell sorting
  • Lipid
  • Single cell sorting

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