Abstract
A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIPY 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to grow after single cell sorting using FACS, Nile Red stained cells failed to recover from sorting. In addition, a comprehensive study was performed to establish a quantitative baseline for future studies for either lipid accumulation and/or microalgal growth by measuring various parameters such as cell count, size, fatty acid contents/composition, and optical/confocal images of the wild type and mutant.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 30-37 |
| Number of pages | 8 |
| Journal | Bioresource Technology |
| Volume | 138 |
| DOIs | |
| State | Published - Jun 2013 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science, and Technology ( ABC-2010-0029728 and ABC-2012-053891 ). N. Velmurugan was supported by the BK21 Post-Doctoral Research Fund and Min S. Park was supported by the Brain Pool Program funded by the Korean Federation of Science and Technology Societies Grant by Korea Government (MEST, Basic Research Promotion Fund).
Keywords
- BODIPY 505/515
- Chlamydomonas reinhardtii
- Fluorescence-activated cell sorting
- Lipid
- Single cell sorting