Evaluation of an inhibitor assay to determine serum isoamylase distribution

Carol Ellis, Donald F. Koehler, John H. Eckfeldt, Michael D. Levitt

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We tested a technique to distinguish salivary from pancreatic isoamylase using a wheat protein which inhibits salivary isoamylase. The inhibitor technique accurately reflected the preponderance of pancreatic or salivary isoamylase in sera which had been "spiked" with human pancreatic or salivary isoamylase. Comparison of the results of either cellulose acetate electrophoresis or isoelectric focusing showed an excellent correlation (r=0.99) in 43 hyperamylasemic sera which did not contain macroamylase. Normal values (1 sd) of total serum amylase activity and the upper limit of normal for serum pancreatic isoamylase was 166 IU/liter. The inhibitor assay provides a simple and accurate means of differentiating salivary from pancreatic hyperamylasemia.

Original languageEnglish (US)
Pages (from-to)897-901
Number of pages5
JournalDigestive Diseases and Sciences
Issue number10
StatePublished - Oct 1 1982

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