TY - JOUR
T1 - Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses
AU - Magnarelli, Louis A.
AU - Ijdo, Jacob W.
AU - Van Andel, Amy E.
AU - Wu, Caiyun
AU - Fikrig, Erol
PY - 2001/1
Y1 - 2001/1
N2 - Objective - To develop and evaluate a polyvalent ELISA incorporating a highly specific recombinant antigen (p44) for diagnosis of granulocytic ehrlichiosis in dogs and horses. Animals - 32 dogs and 43 horses. Procedure - Results of the ELISA were compared with results of indirect fluorescent antibody (IFA) staining and western immunoblotting incorporating whole-cell antigen. Results - For the canine and equine samples, percentages of samples with positive IFA staining, western immunoblotting, and ELISA results were similar. For 29 (91%) canine samples and 30 (70%) equine samples, results of IFA staining, western immunoblotting, and the ELISA were in complete agreement. Results of the ELISA for 3 canine serum samples known to contain antibodies to Ehrlichia canis and 12 equine serum samples known to contain antibodies to E risticii were negative. Conclusions and Clinical Relevance - Results of the present study suggest that a polyvalent ELISA incorporating a recombinant p44 antigen is suitable for detecting antibodies to E equi in dogs and horses.
AB - Objective - To develop and evaluate a polyvalent ELISA incorporating a highly specific recombinant antigen (p44) for diagnosis of granulocytic ehrlichiosis in dogs and horses. Animals - 32 dogs and 43 horses. Procedure - Results of the ELISA were compared with results of indirect fluorescent antibody (IFA) staining and western immunoblotting incorporating whole-cell antigen. Results - For the canine and equine samples, percentages of samples with positive IFA staining, western immunoblotting, and ELISA results were similar. For 29 (91%) canine samples and 30 (70%) equine samples, results of IFA staining, western immunoblotting, and the ELISA were in complete agreement. Results of the ELISA for 3 canine serum samples known to contain antibodies to Ehrlichia canis and 12 equine serum samples known to contain antibodies to E risticii were negative. Conclusions and Clinical Relevance - Results of the present study suggest that a polyvalent ELISA incorporating a recombinant p44 antigen is suitable for detecting antibodies to E equi in dogs and horses.
UR - http://www.scopus.com/inward/record.url?scp=0035227797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035227797&partnerID=8YFLogxK
U2 - 10.2460/ajvr.2001.62.29
DO - 10.2460/ajvr.2001.62.29
M3 - Review article
C2 - 11197555
AN - SCOPUS:0035227797
SN - 0002-9645
VL - 62
SP - 29
EP - 32
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 1
ER -