Evaluation of a hepatocyte‐entrapment hollow fiber bioreactor: A potential bioartificial liver

Scott L. Nyberg, Russel A. Shatford, Madhusudan V. Peshwa, James G. White, Frank B. Cerra, Wei-Shou Hu

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

We have developed a hepatocyte entrapment hollow fiber bioreactor for potential use as a bioartificial liver. Hepatocytes were entrapped in collagen gel inside the lumen of the hollow fibers. Medium was perfused through the intraluminal region after contraction of the hepatocyte‐entrapment gel. Another medium stream, comparable to the patient's blood during clinical application, passed through the extracapillary space. Viability of hepatocytes remained high after 5 days as judged by the rate of oxygen uptake and viability staining. Urea and albumin synthetic activities were also sustained. Transmission electron microscopic examination demonstrated normal ultrastructural integrity of hepatocytes in such a bioreactor. With its sort‐term, extracorporeal support of acute liver failure, the current bioreactor warrants further investigation. © 1993 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)194-203
Number of pages10
JournalBiotechnology and Bioengineering
Volume41
Issue number2
DOIs
StatePublished - Jan 20 1993

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Artificial Liver
Bioreactors
Liver
Hepatocytes
Fibers
Gels
Acute Liver Failure
Nuclear Family
Collagen
Urea
Albumins
Microscopic examination
Blood
Electrons
Staining and Labeling
Oxygen

Keywords

  • artificial liver
  • bioreactor
  • cell entrapment‐liver, artificial
  • hepatocytes

Cite this

Evaluation of a hepatocyte‐entrapment hollow fiber bioreactor : A potential bioartificial liver. / Nyberg, Scott L.; Shatford, Russel A.; Peshwa, Madhusudan V.; White, James G.; Cerra, Frank B.; Hu, Wei-Shou.

In: Biotechnology and Bioengineering, Vol. 41, No. 2, 20.01.1993, p. 194-203.

Research output: Contribution to journalArticle

Nyberg, Scott L. ; Shatford, Russel A. ; Peshwa, Madhusudan V. ; White, James G. ; Cerra, Frank B. ; Hu, Wei-Shou. / Evaluation of a hepatocyte‐entrapment hollow fiber bioreactor : A potential bioartificial liver. In: Biotechnology and Bioengineering. 1993 ; Vol. 41, No. 2. pp. 194-203.
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