Evaluation of a concentration procedure to detect infectious pancreatic necrosis virus in water

S. Maheshkumar, Sagar M Goyal, Philp P. Economon

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A virus adsorption-elution (viradel) method for use with positively charged micro-porous filters was previously developed in our laboratory to concentrate waterborne infectious pancreatic necrosis virus (IPNV). In the present study, this method was evaluated for the detection of IPNV in water of laboratory aquaria containing IPNV-infected rainbow trout Onchorhynchus mykiss. Waterborne IPNV samples concentrated by the viradel method were compared with samples of sediment, fish tissue homogenate, and untreated water io determine how much the viradel method could improve rapidity and efficiency of virus detection. Results of three separate experiments indicated a significant, positive correlation between the virus titers in fish tissue and in the viradel method samples and between the sediment and untreated water samples.

Original languageEnglish (US)
Pages (from-to)58-62
Number of pages5
JournalJournal of Aquatic Animal Health
Volume4
Issue number1
DOIs
StatePublished - Jan 1992

Bibliographical note

Funding Information:
This work (contribution number 263) is the result of research sponsored by the Minnesota Sea Grant College Program supported by the National Ocean and Atmospheric Administration Office of Sea Grant, Department of Commerce (grant NA86AA-D-SG112, project R/NP-3). The tech- nical assistance of Benjamin Kimmel is gratefully acknowledged.

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