Evaluation by bone scintigraphy of osteogenic activity of commercial bioceramics (porous β-TCP and HAp particles) subcutaneously implanted in rats

Hitoshi Nakayama, Tomoyuki Kawase, Hiroyuki Kogami, Kazuhiro Okuda, Hikaru Inoue, Takaaki Oda, Kazuhide Hayama, Makoto Tsuchimochi, Larry F. Wolff

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Osteogenic potential of biomaterials used in bone regenerative therapy has been mainly examined in an animal-implantation study. We have here evaluated the applicability of bone scintigraphy in imaging ectopic bone formation, especially its initial phase, by β-tricalcium phosphate (β-TCP) particles that were implanted in rat dorsal subcutaneous tissues. In implanted osteogenic osteosarcoma cells used as a positive control, osteoid formation was found by histological examination and bone scintigraphy using 99mTc- hydroxymethyl diphosphonate (HMDP) at 2 and 3 weeks post-implantation, respectively, while the microfocuscomputed tomography (μCT) system required further mineralization, which occurred at 4 weeks. Implantation of β-TCP particles alone induced only faint biomineralization inside the particles, which could be microscopically detected by calcein chelation at 2 weeks post-implantation, but not by other histological examinations (e.g., HE staining) or μCT. However, the bone scintigraphy successfully detected this microscopic change at 1 week. Implanted hydroxyapatite (HAp) particles alone used as a negative control did not induce mineralization at microscopic levels, and therefore nothing was detected by either calcein chelation or bone scintigraphy. In conclusion, the bone scintigraphic methodology, although exhibiting less quantitation and resolution, would be applicable as a non-invasive, highly sensitive methodology in detecting the initial, microscopic changes associated with mineralization.

Original languageEnglish (US)
Pages (from-to)751-768
Number of pages18
JournalJournal of Biomaterials Applications
Volume24
Issue number8
DOIs
StatePublished - May 1 2010

Keywords

  • Bone scintigraphy
  • Osteogenic activity
  • β-TCP.
  • μCT

Fingerprint Dive into the research topics of 'Evaluation by bone scintigraphy of osteogenic activity of commercial bioceramics (porous β-TCP and HAp particles) subcutaneously implanted in rats'. Together they form a unique fingerprint.

  • Cite this