Estrogen metabolism in the human lung: Impact of tumorigenesis, smoke, sex and race/ethnicity

Jing Peng, Sibele I. Meireles, Xia Xu, William E. Smith, Michael J. Slifker, Stacy L. Riel, Shumenghui Zhai, Guo Zhang, Xiang Ma, Mindy S. Kurzer, Grace X. Ma, Margie L. Clapper

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Previous data from this group demonstrate that the murine lung metabolizes estrogen. Production of the putative carcinogen 4-hydroxyestrogen (4-OHE) is elevated within the lungs of female vs. male mice and accelerated by tobacco smoke. The goal of this study was to determine if the human lung metabolizes estrogen and evaluate the impact of tumor formation, smoke, sex and race/ethnicity on metabolism. Urine and lung tissue (normal, tumor) were obtained from 49 non-small cell lung cancer patients. Healthy postmenopausal Caucasian (n = 19) and Chinese (n = 20) American women (never-smokers) donated urine. Quantitative RT-PCR analyses indicate that multiple estrogen synthesis and metabolism genes are expressed in human bronchoalveolar cells. Estrogen and its metabolites were measured in lung tissue and urine using liquid chromatography/tandem mass spectrometry. Wilcoxon rank tests were used for statistical comparisons. E1, E2, E3 and estrogen metabolites 2-OHE1, 2-OHE2, 4-OHE1, 4-OHE2, 2-OME1 and 2-OME2 were detected at higher levels in tumor vs. adjacent normal tissue and in women vs. men (P < 0.05). The proportion of 4-OHEs was higher in tumors than in normal lung tissue (P < 0.05), and elevated in normal tissue from currentvs. never-smoking women (P = 0.006); similar trends were observed in urine. The proportion of 4-OHEs in the urine of postmenopausal Chinese American women was 1.8- fold higher than that of Caucasian women (P = 0.015). These data indicate that estrogen metabolites are present in the human lung. A shift towards 4-hydroxylation during lung tumorigenesis may contribute to the risk conferred by smoking, sex or race/ethnicity.

Original languageEnglish (US)
Pages (from-to)106778-106789
Number of pages12
JournalOncotarget
Volume8
Issue number63
DOIs
StatePublished - Jan 1 2017

Fingerprint

Smoke
Estrogens
Carcinogenesis
Lung
Urine
Neoplasms
Smoking
Asian Americans
Hydroxylation
Nonparametric Statistics
Tandem Mass Spectrometry
Non-Small Cell Lung Carcinoma
Liquid Chromatography
Carcinogens
Tobacco
Polymerase Chain Reaction
Genes

Keywords

  • 4-hydroxy estrogen
  • Estrogen metabolism
  • Never-smoking Chinese women
  • Non-small cell lung cancer
  • Tobacco smoke

Cite this

Peng, J., Meireles, S. I., Xu, X., Smith, W. E., Slifker, M. J., Riel, S. L., ... Clapper, M. L. (2017). Estrogen metabolism in the human lung: Impact of tumorigenesis, smoke, sex and race/ethnicity. Oncotarget, 8(63), 106778-106789. https://doi.org/10.18632/oncotarget.22269

Estrogen metabolism in the human lung : Impact of tumorigenesis, smoke, sex and race/ethnicity. / Peng, Jing; Meireles, Sibele I.; Xu, Xia; Smith, William E.; Slifker, Michael J.; Riel, Stacy L.; Zhai, Shumenghui; Zhang, Guo; Ma, Xiang; Kurzer, Mindy S.; Ma, Grace X.; Clapper, Margie L.

In: Oncotarget, Vol. 8, No. 63, 01.01.2017, p. 106778-106789.

Research output: Contribution to journalArticle

Peng, J, Meireles, SI, Xu, X, Smith, WE, Slifker, MJ, Riel, SL, Zhai, S, Zhang, G, Ma, X, Kurzer, MS, Ma, GX & Clapper, ML 2017, 'Estrogen metabolism in the human lung: Impact of tumorigenesis, smoke, sex and race/ethnicity', Oncotarget, vol. 8, no. 63, pp. 106778-106789. https://doi.org/10.18632/oncotarget.22269
Peng J, Meireles SI, Xu X, Smith WE, Slifker MJ, Riel SL et al. Estrogen metabolism in the human lung: Impact of tumorigenesis, smoke, sex and race/ethnicity. Oncotarget. 2017 Jan 1;8(63):106778-106789. https://doi.org/10.18632/oncotarget.22269
Peng, Jing ; Meireles, Sibele I. ; Xu, Xia ; Smith, William E. ; Slifker, Michael J. ; Riel, Stacy L. ; Zhai, Shumenghui ; Zhang, Guo ; Ma, Xiang ; Kurzer, Mindy S. ; Ma, Grace X. ; Clapper, Margie L. / Estrogen metabolism in the human lung : Impact of tumorigenesis, smoke, sex and race/ethnicity. In: Oncotarget. 2017 ; Vol. 8, No. 63. pp. 106778-106789.
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