Estradiol's beneficial effect on murine muscle function is independent of muscle activity

Sarah M. Greising, Kristen A. Baltgalvis, Allison M. Kosir, Amy L. Moran, Gordon L. Warren, Dawn A. Lowe

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


Estradiol (E2) deficiency decreases muscle strength and wheel running in female mice. It is not known if the muscle weakness results directly from the loss of E2 or indirectly from mice becoming relatively inactive with presumably diminished muscle activity. The first aim of this study was to determine if cage activities of ovariectomized mice with and without E2 treatment differ. Ovariectomized mice were 19-46% less active than E2-replaced mice in terms of ambulation, jumping, and time spent being active (P ≤ 0.033). After E2-deficient mice were found to have low cage activities, the second aim was to determine if E2 is beneficial to muscle contractility, independent of physical activities by the mouse or its hindlimb muscles. Adult, female mice were ovariectomized or sham-operated and randomized to receive E2 or placebo and then subjected to conditions that should maintain physical and muscle activity at a constant low level. After 2 wk of hindlimb suspension or unilateral tibial nerve transection, muscle contractile function was assessed. Soleus muscles of hindlimbsuspended ovariectomized mice generated 31% lower normalized (relative to muscle contractile protein content) maximal isometric force than suspended mice with intact ovaries (P ≤ 0.049). Irrespective of whether the soleus muscle was innervated, muscles from ovariectomized mice generated ∼20% lower absolute and normalized maximal isometric forces, as well as power, than E 2-replaced mice (P ≤ 0.004). In conclusion, E2 affects muscle force generation, even when muscle activity is equalized.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalJournal of applied physiology
Issue number1
StatePublished - Jan 2011


  • Estrogen
  • Hindlimb suspension
  • Ovariectomy
  • Physical activity
  • Tibial nerve transection


Dive into the research topics of 'Estradiol's beneficial effect on murine muscle function is independent of muscle activity'. Together they form a unique fingerprint.

Cite this