Estimation of the relative fertilizing ability of frozen chicken spermatozoa using a heterospermic competition method

A. Tajima, E. F. Graham, D. M. Hawkins

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Semen was collected from Rhode Island Red and White Leghorn roosters. After adjusting the sperm number from both breeds, half of the semen was frozen-thawed in the presence of glycerol. Frozen and unfrozen semen from both breeds was mixed 1:1 with all four possible combinations and inseminated into Rhode Island Red hens. Feather colour of chicks was used to determine which breed fertilized the eggs. Results showed that sperm cells retained 19.7% [95% confidence interval = (12.8, 30.4)] of the relative fertilizing ability after freezing. Furthermore, Rhode Island Red spermatozoa had 1.5 times [95% confidence interval = (1.1, 2.0)] higher relative fertilizing ability than did White Leghorn spermatozoa. The heterospermic competition assay method is a powerful tool for estimating the relative fertilizing ability of the sperm cells.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of reproduction and fertility
Volume85
Issue number1
DOIs
StatePublished - 1989

Fingerprint Dive into the research topics of 'Estimation of the relative fertilizing ability of frozen chicken spermatozoa using a heterospermic competition method'. Together they form a unique fingerprint.

  • Cite this