Establishment of bisegmented and trisegmented reverse genetics systems to generate recombinant pichindé viruses

Rekha Dhanwani, Qinfeng Huang, Shuiyun Lan, Yanqing Zhou, Junjie Shao, Yuying Liang, Hinh Ly

Research output: ResearchChapter

Abstract

Pichindé virus (PICV), isolated from rice rats in Colombia, South America, is an enveloped arenavirus with a bisegmented RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-dependent RNA polymerase, whereas the small (S) genomic segment encodes the nucleoprotein (NP) and the glycoprotein (GPC). This article describes the successful development of reverse genetics systems to generate recombinant PICV with either a bisegmented or trisegmented genome. We have successfully demonstrated that these systems can generate high-titered and genetically stable replication-competent viruses from plasmid transfection into appropriate cell lines. These systems demonstrate the power and versatility of reverse genetic technology to generate recombinant arenaviruses for use in pathogenesis studies and as new viral vaccine vectors.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages247-253
Number of pages7
Volume1604
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1604
ISSN (Print)1064-3745

Fingerprint

Pichinde virus
Arenavirus
Reverse Genetics
Genome
Viral Vaccines
Sigmodontinae
RNA Replicase
Nucleoproteins
Colombia
South America
Virus Replication
Transfection
Glycoproteins
Plasmids
RNA
Technology
Cell Line
plasma protein Z

Keywords

  • Arenavirus
  • Pichindé virus
  • Reverse genetics

Cite this

Dhanwani, R., Huang, Q., Lan, S., Zhou, Y., Shao, J., Liang, Y., & Ly, H. (2018). Establishment of bisegmented and trisegmented reverse genetics systems to generate recombinant pichindé viruses. In Methods in Molecular Biology (Vol. 1604, pp. 247-253). (Methods in Molecular Biology; Vol. 1604). Humana Press Inc.. DOI: 10.1007/978-1-4939-6981-4_19

Establishment of bisegmented and trisegmented reverse genetics systems to generate recombinant pichindé viruses. / Dhanwani, Rekha; Huang, Qinfeng; Lan, Shuiyun; Zhou, Yanqing; Shao, Junjie; Liang, Yuying; Ly, Hinh.

Methods in Molecular Biology. Vol. 1604 Humana Press Inc., 2018. p. 247-253 (Methods in Molecular Biology; Vol. 1604).

Research output: ResearchChapter

Dhanwani, R, Huang, Q, Lan, S, Zhou, Y, Shao, J, Liang, Y & Ly, H 2018, Establishment of bisegmented and trisegmented reverse genetics systems to generate recombinant pichindé viruses. in Methods in Molecular Biology. vol. 1604, Methods in Molecular Biology, vol. 1604, Humana Press Inc., pp. 247-253. DOI: 10.1007/978-1-4939-6981-4_19
Dhanwani R, Huang Q, Lan S, Zhou Y, Shao J, Liang Y et al. Establishment of bisegmented and trisegmented reverse genetics systems to generate recombinant pichindé viruses. In Methods in Molecular Biology. Vol. 1604. Humana Press Inc.2018. p. 247-253. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-4939-6981-4_19
Dhanwani, Rekha ; Huang, Qinfeng ; Lan, Shuiyun ; Zhou, Yanqing ; Shao, Junjie ; Liang, Yuying ; Ly, Hinh. / Establishment of bisegmented and trisegmented reverse genetics systems to generate recombinant pichindé viruses. Methods in Molecular Biology. Vol. 1604 Humana Press Inc., 2018. pp. 247-253 (Methods in Molecular Biology).
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