The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.
Bibliographical noteFunding Information:
The authors thank Dr. T. O'Brien for review of pathological materials and for helpful discussions, and Dr. A. C. Avery for performance of the PARR experiments. The authors also acknowledge donations to the Animal Cancer Care and Research Program of the University of Minnesota that helped support this project. This study was supported in part by the AKC Canine Health Foundation (grant number 1759), the National Canine Cancer Foundation (grant number DM06CO-003) and the NIH Comprehensive Cancer Center Support Grant (grant number P30 CA077598).
© 2016 Elsevier Ltd
- enteropathy-associated T-cell lymphoma
- immunodeficient mouse model
- patient-derived tumour xenograft