Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type

K. S. Im, Jong Hyuk Kim, A. J. Graef, Ingrid Cornax, Davis Seelig, Gerard O'Sullivan, R. C. Kovi, Jaime Modiano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalJournal of Comparative Pathology
Volume156
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Enteropathy-Associated T-Cell Lymphoma
digestive system diseases
lymphoma
Heterografts
Canidae
T-lymphocytes
neoplasms
dogs
Neoplasms
cells
T-Cell Lymphoma
mice
pathogenesis
Golden Retriever
Antigen Receptors
Nude Mice
spleen
antigens
Spleen
receptors

Keywords

  • dog
  • enteropathy-associated T-cell lymphoma
  • immunodeficient mouse model
  • patient-derived tumour xenograft

Cite this

Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type. / Im, K. S.; Kim, Jong Hyuk; Graef, A. J.; Cornax, Ingrid; Seelig, Davis; O'Sullivan, Gerard; Kovi, R. C.; Modiano, Jaime.

In: Journal of Comparative Pathology, Vol. 156, No. 1, 01.01.2017, p. 37-41.

Research output: Contribution to journalArticle

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AB - The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.

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