Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type

K. S. Im; J. H. Kim; A. J. Graef; I. Cornax; D. M. Seelig; M. G. O'Sullivan; R. C. Kovi; J. F. Modiano

doi:10.1016/j.jcpa.2016.11.271

Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type

K. S. Im, J. H. Kim, A. J. Graef, I. Cornax, D. M. Seelig, M. G. O'Sullivan, R. C. Kovi, J. F. Modiano

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.

Original language	English (US)
Pages (from-to)	37-41
Number of pages	5
Journal	Journal of Comparative Pathology
Volume	156
Issue number	1
DOIs	https://doi.org/10.1016/j.jcpa.2016.11.271
State	Published - Jan 1 2017

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Publisher Copyright:
© 2016 Elsevier Ltd

Keywords

dog
enteropathy-associated T-cell lymphoma
immunodeficient mouse model
patient-derived tumour xenograft

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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http://europepmc.org/articles/pmc5292073

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title = "Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type",

abstract = "The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.",

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AU - Im, K. S.

AU - Kim, J. H.

AU - Graef, A. J.

AU - Cornax, I.

AU - Seelig, D. M.

AU - O'Sullivan, M. G.

AU - Kovi, R. C.

AU - Modiano, J. F.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.

AB - The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.

KW - dog

KW - enteropathy-associated T-cell lymphoma

KW - immunodeficient mouse model

KW - patient-derived tumour xenograft

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Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type

Abstract

Bibliographical note

Keywords

UN SDGs

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