Escape from telomere-driven crisis is DNA ligase III dependent

Rhiannon E. Jones, Sehyun Oh, Julia W. Grimstead, Jacob Zimbric, Laureline Roger, Nicole H. Heppel, Kevin E. Ashelford, Kate Liddiard, Eric A. Hendrickson, Duncan M. Baird

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Abstract

Short dysfunctional telomeres are capable of fusion, generating dicentric chromosomes and initiating breakage-fusion-bridge cycles. Cells that escape the ensuing cellular crisis exhibit large-scale genomic rearrangements that drive clonal evolution and malignant progression. We demonstrate that there is an absolute requirement for fully functional DNA ligase III (LIG3), but not ligase IV (LIG4), to facilitate the escape from a telomere-driven crisis. LIG3- and LIG4-dependent alternative (A) and classical (C) nonhomologous end-joining (NHEJ) pathways were capable of mediating the fusion of shortdysfunctional telomeres, both displaying characteristic patterns of microhomology and deletion. Cells that failed to escape crisis exhibited increased proportions of C-NHEJ-mediated interchromosomal fusions, whereas those that escaped displayed increased proportions of intrachromosomal fusions. We propose that the balance between inter- and intrachromosomal telomere fusions dictates the ability of human cells to escape crisis and is influenced by the relative activities of A- and C-NHEJ at short dysfunctional telomeres.

Original languageEnglish (US)
Pages (from-to)1063-1076
Number of pages14
JournalCell reports
Volume8
Issue number4
DOIs
StatePublished - Aug 21 2014

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DNA Ligases
Telomere
Fusion reactions
Joining
Clonal Evolution
Chromosome Breakage
Ligases
Chromosomes
DNA Ligase ATP
Cells

Cite this

Jones, R. E., Oh, S., Grimstead, J. W., Zimbric, J., Roger, L., Heppel, N. H., ... Baird, D. M. (2014). Escape from telomere-driven crisis is DNA ligase III dependent. Cell reports, 8(4), 1063-1076. https://doi.org/10.1016/j.celrep.2014.07.007

Escape from telomere-driven crisis is DNA ligase III dependent. / Jones, Rhiannon E.; Oh, Sehyun; Grimstead, Julia W.; Zimbric, Jacob; Roger, Laureline; Heppel, Nicole H.; Ashelford, Kevin E.; Liddiard, Kate; Hendrickson, Eric A.; Baird, Duncan M.

In: Cell reports, Vol. 8, No. 4, 21.08.2014, p. 1063-1076.

Research output: Contribution to journalArticle

Jones, RE, Oh, S, Grimstead, JW, Zimbric, J, Roger, L, Heppel, NH, Ashelford, KE, Liddiard, K, Hendrickson, EA & Baird, DM 2014, 'Escape from telomere-driven crisis is DNA ligase III dependent', Cell reports, vol. 8, no. 4, pp. 1063-1076. https://doi.org/10.1016/j.celrep.2014.07.007
Jones RE, Oh S, Grimstead JW, Zimbric J, Roger L, Heppel NH et al. Escape from telomere-driven crisis is DNA ligase III dependent. Cell reports. 2014 Aug 21;8(4):1063-1076. https://doi.org/10.1016/j.celrep.2014.07.007
Jones, Rhiannon E. ; Oh, Sehyun ; Grimstead, Julia W. ; Zimbric, Jacob ; Roger, Laureline ; Heppel, Nicole H. ; Ashelford, Kevin E. ; Liddiard, Kate ; Hendrickson, Eric A. ; Baird, Duncan M. / Escape from telomere-driven crisis is DNA ligase III dependent. In: Cell reports. 2014 ; Vol. 8, No. 4. pp. 1063-1076.
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