The effect of the thiol‐oxidizing agent diamide on erythrocyte (Ca+2+Mg+2)‐ATPase activity was measured in normal and glucose‐6‐phosphate‐dehydrogenase‐deficient (G6PD−) cells. Although the enzyme activity before the oxidative stress was similar in both groups, diamide induced a markedly greater inhibition in the enzyme activity in the G6PD− cells than in the normal controls. These data indicate dependence of erythrocyte (Ca+2+Mg+2)‐ATPase, in part, on the redox status of the cell. The increased vulnerability of (Ca+2+Mg+2)ATPase to oxidative stress in G6PD− may be of pathophysiological relevance to their premature destruction in oxidant‐induced hemolysis.
- adenosine triphosphatase
- glucose‐6‐phosphate dehydrogenase