TY - JOUR
T1 - Erratum
T2 - Chronic Alcohol Ingestion Increases Mortality and Organ Injury in a Murine Model of Septic Peritonitis (PLoS ONE (2013) 8:5 (e62792) DOI: 10.1371/journal.pone.0062792)
AU - Yoseph, Benyam P.
AU - Breed, Elise
AU - Overgaard, Christian E.
AU - Ward, Christina J.
AU - Liang, Zhe
AU - Wagener, Maylene E.
AU - Lexcen, Daniel R.
AU - Lusczek, Elizabeth R.
AU - Beilman, Greg J.
AU - Burd, Eileen M.
AU - Farris, Alton B.
AU - Guidot, David M.
AU - Koval, Michael
AU - Ford, Mandy L.
AU - Coopersmith, Craig M.
N1 - Publisher Copyright:
© 2020 Yoseph et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2020/9
Y1 - 2020/9
N2 - After publication of this article [1], concerns were raised about the following results in Fig 5: • β-actin data shown in the Water (lanes 1, 2) and Alcohol (lane 1) panels in Fig 5B appear similar to β-actin data shown in the Water (lanes 1, 2) and Alcohol (lane 2) panels, respectively, of Fig 5C. • In Fig 5D, Claudin-2 panel, the same data appear to be presented for the Water and Alcohol groups. The authors noted that an error was made in preparing the Claudin-2 panel for the Alcohol group in Fig 5D. Regarding the β-actin duplications, as shown in S1 File, the duplicated β-actin data and corresponding ZO-1 and Occludin data correspond to the same lanes in all three blots. For other experiments in the same series, laboratory records indicate that the authors consistently ran the same protein samples on parallel blots probed with ZO-1, Occludin, and β-actin. Thus, the authors noted that the same β-actin control data likely apply to ZO-1 and Occludin experiments reported in Fig 5B and 5C. The authors apologize that this aspect of the experimental design was not clearly described in the original article. A corrected version of Fig 5 is provided here. The full-sized original blots are no longer available, but images showing all experimental lanes of the original blots are provided in S1 File, and the raw quantification data are in S2 File. Raw data are available for all other data in the manuscript except source flow cytometry plots for Figs 8 and 10. The authors apologize for the errors in the published article.
AB - After publication of this article [1], concerns were raised about the following results in Fig 5: • β-actin data shown in the Water (lanes 1, 2) and Alcohol (lane 1) panels in Fig 5B appear similar to β-actin data shown in the Water (lanes 1, 2) and Alcohol (lane 2) panels, respectively, of Fig 5C. • In Fig 5D, Claudin-2 panel, the same data appear to be presented for the Water and Alcohol groups. The authors noted that an error was made in preparing the Claudin-2 panel for the Alcohol group in Fig 5D. Regarding the β-actin duplications, as shown in S1 File, the duplicated β-actin data and corresponding ZO-1 and Occludin data correspond to the same lanes in all three blots. For other experiments in the same series, laboratory records indicate that the authors consistently ran the same protein samples on parallel blots probed with ZO-1, Occludin, and β-actin. Thus, the authors noted that the same β-actin control data likely apply to ZO-1 and Occludin experiments reported in Fig 5B and 5C. The authors apologize that this aspect of the experimental design was not clearly described in the original article. A corrected version of Fig 5 is provided here. The full-sized original blots are no longer available, but images showing all experimental lanes of the original blots are provided in S1 File, and the raw quantification data are in S2 File. Raw data are available for all other data in the manuscript except source flow cytometry plots for Figs 8 and 10. The authors apologize for the errors in the published article.
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U2 - 10.1371/journal.pone.0239568
DO - 10.1371/journal.pone.0239568
M3 - Comment/debate
C2 - 32941549
AN - SCOPUS:85091192646
SN - 1932-6203
VL - 15
JO - PloS one
JF - PloS one
IS - 9
M1 - e0239568
ER -